A versatile system based on substrate formulation using agroindustrial wastes for protease production by Aspergillus niger under solid state fermentation

Ruann Janser Soares de Castro, André Ohara, Tânia Goia Nishide, Marcela Pavan Bagagli, Fernanda Furlan Gonçalves Dias, Hélia Harumi Sato

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

This study aimed to evaluate the substrate formulation using different agroindustrial wastes (wheat bran, soybean meal, cottonseed meal and orange peel) as a tool to purpose a versatile system for protease production by Aspergillus niger LBA 02 under solid state fermentation. The results showed interesting synergistic and antagonistic effects between the agroindustrial wastes during the protease production. The highest protease activity was found using the medium containing wheat bran (0.50) and soybean meal (0.50), reaching 262.78Ug-1 after 48h fermentation. The most prominent synergetic effects were observed on fermentations performed using the medium composed by the four agroindustrial wastes in equal proportions at 48 and 72h fermentation, reaching increases of 33.7%, 7.6%, 30.8% and 581.7% and 11.6%, 131.4%, 69.5% and 547.0%, respectively, in protease production as compared to the individual substrates. From these results was also possible to establish the use of at least three different culture media formulations with a maximum protease production above 260Ug-1, making it a versatile and efficient system for protease production and that can be easily applicable to other groups of enzymes.

Original languageEnglish (US)
Pages (from-to)678-684
Number of pages7
JournalBiocatalysis and Agricultural Biotechnology
Volume4
Issue number4
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

  • Agroindustrial wastes
  • Protease
  • Solid state fermentation
  • Statistical mixture design

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