A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

Benjamin P. Duckworth; Yan Chen; James W. Wollack; Yuk Sham; Joachim D. Mueller; T. Andrew Taton; Mark D. Distefano

doi:10.1002/anie.200701942

A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

Benjamin P. Duckworth
, Yan Chen
, James W. Wollack
, Yuk Sham
, Joachim D. Mueller
, T. Andrew Taton
, Mark D. Distefano

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.

Original language	English (US)
Pages (from-to)	8819-8822
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	46
DOIs	https://doi.org/10.1002/anie.200701942
State	Published - 2007

Keywords

Click reactions
Enzymes
Nanostructures
Protein labeling
Protein structures

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10.1002/anie.200701942

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title = "A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures",

abstract = "(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.",

keywords = "Click reactions, Enzymes, Nanostructures, Protein labeling, Protein structures",

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year = "2007",

doi = "10.1002/anie.200701942",

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volume = "46",

pages = "8819--8822",

journal = "Angewandte Chemie - International Edition",

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AU - Duckworth, Benjamin P.

AU - Chen, Yan

AU - Wollack, James W.

AU - Sham, Yuk

AU - Mueller, Joachim D.

AU - Taton, T. Andrew

AU - Distefano, Mark D.

PY - 2007

Y1 - 2007

N2 - (Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.

AB - (Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.

KW - Click reactions

KW - Enzymes

KW - Nanostructures

KW - Protein labeling

KW - Protein structures

UR - https://www.scopus.com/pages/publications/36749055668

UR - https://www.scopus.com/pages/publications/36749055668#tab=citedBy

U2 - 10.1002/anie.200701942

DO - 10.1002/anie.200701942

M3 - Article

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AN - SCOPUS:36749055668

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 46

ER -

A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

Abstract

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