A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race

Pablo D. Olivera; Dolors Villegas; Carlos Cantero-Martínez; Les J. Szabo; Matthew N. Rouse; Douglas G. Luster; Radhika Bartaula; Marta S. Lopes; Yue Jin

doi:10.1111/ppa.13530

A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race

Pablo D. Olivera, Dolors Villegas, Carlos Cantero-Martínez, Les J. Szabo, Matthew N. Rouse, Douglas G. Luster, Radhika Bartaula, Marta S. Lopes, Yue Jin

Plant Pathology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a re-emerging disease, posing a threat to wheat production. In Spain, stem rust has been rarely detected since the 1970s, but infection was observed in wheat fields in 2018. We analysed six stem rust samples collected in Rota, Cádiz province and one from Monteagudo del Castillo, Teruel province. All the samples from Rota were typed as race TKTTF, whereas the sample from Monteagudo del Castillo, collected in a wheat field adjacent to barberry bushes, was typed as race TKHBK. This race has a unique and significant virulence combination that includes virulence to Sr31, Sr33, Sr53 and Sr59, and is avirulent to Rusty, a durum line developed for universal susceptibility to the wheat stem rust pathogen. TKHBK is the first race outside the Ug99 race group with virulence to Sr31 and the first known race with virulence to Sr59. Genotyping studies indicate that race TKHBK does not belong to the Ug99 or TKTTF race groups and constitutes a previously unknown lineage. Two hundred bread and durum wheat cultivars and breeding lines from Spain were evaluated against TKHBK, TKTTF, and six additional races. Resistance was observed to all the races evaluated. Molecular markers confirmed the presence of Sr7a, Sr24, Sr31, Sr38 and Sr57 in bread wheat, and Sr13 in durum wheat. The re-emergence of wheat stem rust in Spain and the occurrence of unique virulences underscore the need to continue surveying and monitoring this disease.

Original language	English (US)
Pages (from-to)	873-889
Number of pages	17
Journal	Plant Pathology
Volume	71
Issue number	4
DOIs	https://doi.org/10.1111/ppa.13530
State	Published - May 2022

Bibliographical note

Funding Information:
We thank Melissa Carter (USDA‐ARS Foreign Disease‐Weed Science Research Unit), Jerry Johnson (USDA‐ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture–Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015‐00072‐C03‐01 of INIA and PID2020‐118650RR‐C31 of Spanish National Plan of Research, Spain.

Funding Information:
We thank Melissa Carter (USDA-ARS Foreign Disease-Weed Science Research Unit), Jerry Johnson (USDA-ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture?Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015-00072-C03-01 of INIA and PID2020-118650RR-C31 of Spanish National Plan of Research, Spain.

Publisher Copyright:
© 2022 British Society for Plant Pathology. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

Keywords

Puccinia graminis f. sp. tritici
gene postulation
stem rust resistance gene

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10.1111/ppa.13530

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@article{e0c17abae8d84c1384e66154c7ecc307,

title = "A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race",

abstract = "Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a re-emerging disease, posing a threat to wheat production. In Spain, stem rust has been rarely detected since the 1970s, but infection was observed in wheat fields in 2018. We analysed six stem rust samples collected in Rota, C{\'a}diz province and one from Monteagudo del Castillo, Teruel province. All the samples from Rota were typed as race TKTTF, whereas the sample from Monteagudo del Castillo, collected in a wheat field adjacent to barberry bushes, was typed as race TKHBK. This race has a unique and significant virulence combination that includes virulence to Sr31, Sr33, Sr53 and Sr59, and is avirulent to Rusty, a durum line developed for universal susceptibility to the wheat stem rust pathogen. TKHBK is the first race outside the Ug99 race group with virulence to Sr31 and the first known race with virulence to Sr59. Genotyping studies indicate that race TKHBK does not belong to the Ug99 or TKTTF race groups and constitutes a previously unknown lineage. Two hundred bread and durum wheat cultivars and breeding lines from Spain were evaluated against TKHBK, TKTTF, and six additional races. Resistance was observed to all the races evaluated. Molecular markers confirmed the presence of Sr7a, Sr24, Sr31, Sr38 and Sr57 in bread wheat, and Sr13 in durum wheat. The re-emergence of wheat stem rust in Spain and the occurrence of unique virulences underscore the need to continue surveying and monitoring this disease.",

keywords = "Puccinia graminis f. sp. tritici, gene postulation, stem rust resistance gene",

author = "Olivera, {Pablo D.} and Dolors Villegas and Carlos Cantero-Mart{\'i}nez and Szabo, {Les J.} and Rouse, {Matthew N.} and Luster, {Douglas G.} and Radhika Bartaula and Lopes, {Marta S.} and Yue Jin",

note = "Funding Information: We thank Melissa Carter (USDA‐ARS Foreign Disease‐Weed Science Research Unit), Jerry Johnson (USDA‐ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture–Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015‐00072‐C03‐01 of INIA and PID2020‐118650RR‐C31 of Spanish National Plan of Research, Spain. Funding Information: We thank Melissa Carter (USDA-ARS Foreign Disease-Weed Science Research Unit), Jerry Johnson (USDA-ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture?Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015-00072-C03-01 of INIA and PID2020-118650RR-C31 of Spanish National Plan of Research, Spain. Publisher Copyright: {\textcopyright} 2022 British Society for Plant Pathology. This article has been contributed to by US Government employees and their work is in the public domain in the USA.",

year = "2022",

month = may,

doi = "10.1111/ppa.13530",

language = "English (US)",

volume = "71",

pages = "873--889",

journal = "Plant Pathology",

issn = "0032-0862",

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T1 - A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race

AU - Olivera, Pablo D.

AU - Villegas, Dolors

AU - Cantero-Martínez, Carlos

AU - Szabo, Les J.

AU - Rouse, Matthew N.

AU - Luster, Douglas G.

AU - Bartaula, Radhika

AU - Lopes, Marta S.

AU - Jin, Yue

N1 - Funding Information: We thank Melissa Carter (USDA‐ARS Foreign Disease‐Weed Science Research Unit), Jerry Johnson (USDA‐ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture–Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015‐00072‐C03‐01 of INIA and PID2020‐118650RR‐C31 of Spanish National Plan of Research, Spain. Funding Information: We thank Melissa Carter (USDA-ARS Foreign Disease-Weed Science Research Unit), Jerry Johnson (USDA-ARS Cereal Disease Laboratory) and Krista Ristinen (University of Minnesota) for their technical assistance. This research was supported by UK Aid from the British People and the Bill & Melinda Gates Foundation (OPP1133199), and United States Department of Agriculture?Agricultural Research Service. The authors from IRTA also acknowledge the contribution of the CERCA Programme (Generalitat de Catalunya), the project RTA2015-00072-C03-01 of INIA and PID2020-118650RR-C31 of Spanish National Plan of Research, Spain. Publisher Copyright: © 2022 British Society for Plant Pathology. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

PY - 2022/5

Y1 - 2022/5

N2 - Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a re-emerging disease, posing a threat to wheat production. In Spain, stem rust has been rarely detected since the 1970s, but infection was observed in wheat fields in 2018. We analysed six stem rust samples collected in Rota, Cádiz province and one from Monteagudo del Castillo, Teruel province. All the samples from Rota were typed as race TKTTF, whereas the sample from Monteagudo del Castillo, collected in a wheat field adjacent to barberry bushes, was typed as race TKHBK. This race has a unique and significant virulence combination that includes virulence to Sr31, Sr33, Sr53 and Sr59, and is avirulent to Rusty, a durum line developed for universal susceptibility to the wheat stem rust pathogen. TKHBK is the first race outside the Ug99 race group with virulence to Sr31 and the first known race with virulence to Sr59. Genotyping studies indicate that race TKHBK does not belong to the Ug99 or TKTTF race groups and constitutes a previously unknown lineage. Two hundred bread and durum wheat cultivars and breeding lines from Spain were evaluated against TKHBK, TKTTF, and six additional races. Resistance was observed to all the races evaluated. Molecular markers confirmed the presence of Sr7a, Sr24, Sr31, Sr38 and Sr57 in bread wheat, and Sr13 in durum wheat. The re-emergence of wheat stem rust in Spain and the occurrence of unique virulences underscore the need to continue surveying and monitoring this disease.

AB - Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a re-emerging disease, posing a threat to wheat production. In Spain, stem rust has been rarely detected since the 1970s, but infection was observed in wheat fields in 2018. We analysed six stem rust samples collected in Rota, Cádiz province and one from Monteagudo del Castillo, Teruel province. All the samples from Rota were typed as race TKTTF, whereas the sample from Monteagudo del Castillo, collected in a wheat field adjacent to barberry bushes, was typed as race TKHBK. This race has a unique and significant virulence combination that includes virulence to Sr31, Sr33, Sr53 and Sr59, and is avirulent to Rusty, a durum line developed for universal susceptibility to the wheat stem rust pathogen. TKHBK is the first race outside the Ug99 race group with virulence to Sr31 and the first known race with virulence to Sr59. Genotyping studies indicate that race TKHBK does not belong to the Ug99 or TKTTF race groups and constitutes a previously unknown lineage. Two hundred bread and durum wheat cultivars and breeding lines from Spain were evaluated against TKHBK, TKTTF, and six additional races. Resistance was observed to all the races evaluated. Molecular markers confirmed the presence of Sr7a, Sr24, Sr31, Sr38 and Sr57 in bread wheat, and Sr13 in durum wheat. The re-emergence of wheat stem rust in Spain and the occurrence of unique virulences underscore the need to continue surveying and monitoring this disease.

KW - Puccinia graminis f. sp. tritici

KW - gene postulation

KW - stem rust resistance gene

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JO - Plant Pathology

JF - Plant Pathology

IS - 4

ER -

A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race

Abstract

Bibliographical note

Keywords

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