A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain

Stevanus R. Tedjakumala, Jacques Rouquette, Marie Laure Boizeau, Karen A. Mesce, Lucie Hotier, Isabelle Massou, Martin Giurfa

Research output: Contribution to journalArticle

Abstract

Dopamine (DA) plays a fundamental role in insect behavior as it acts both as a general modulator of behavior and as a value system in associative learning where it mediates the reinforcing properties of unconditioned stimuli (US). Here we aimed at characterizing the dopaminergic neurons in the central nervous system of the honey bee, an insect that serves as an established model for the study of learning and memory. We used tyrosine hydroxylase (TH) immunoreactivity (ir) to ensure that the neurons detected synthesize DA endogenously. We found three main dopaminergic clusters, C1–C3, which had been previously described; the C1 cluster is located in a small region adjacent to the esophagus (ES) and the antennal lobe (AL); the C2 cluster is situated above the C1 cluster, between the AL and the vertical lobe (VL) of the mushroom body (MB); the C3 cluster is located below the calyces (CA) of the MB. In addition, we found a novel dopaminergic cluster, C4, located above the dorsomedial border of the lobula, which innervates the visual neuropils of the bee brain. Additional smaller processes and clusters were found and are described. The profuse dopaminergic innervation of the entire bee brain and the specific connectivity of DA neurons, with visual, olfactory and gustatory circuits, provide a foundation for a deeper understanding of how these sensory modules are modulated by DA, and the DA-dependent value-based associations that occur during associative learning.

Original languageEnglish (US)
Article number47
JournalFrontiers in Systems Neuroscience
Volume11
DOIs
StatePublished - Jul 10 2017

Fingerprint

Honey
Bees
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Dopamine
Brain
Learning
Mushroom Bodies
Insects
Neurons
Esophagus
Central Nervous System

Keywords

  • Apis mellifera
  • Dopamine
  • Dopaminergic signaling
  • Neural circuits
  • Neural clusters

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Developmental Neuroscience
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

MeSH PubMed subject areas

  • Journal Article

Cite this

Tedjakumala, S. R., Rouquette, J., Boizeau, M. L., Mesce, K. A., Hotier, L., Massou, I., & Giurfa, M. (2017). A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain. Frontiers in Systems Neuroscience, 11, [47]. DOI: 10.3389/fnsys.2017.00047

A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain. / Tedjakumala, Stevanus R.; Rouquette, Jacques; Boizeau, Marie Laure; Mesce, Karen A.; Hotier, Lucie; Massou, Isabelle; Giurfa, Martin.

In: Frontiers in Systems Neuroscience, Vol. 11, 47, 10.07.2017.

Research output: Contribution to journalArticle

Tedjakumala, SR, Rouquette, J, Boizeau, ML, Mesce, KA, Hotier, L, Massou, I & Giurfa, M 2017, 'A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain' Frontiers in Systems Neuroscience, vol 11, 47. DOI: 10.3389/fnsys.2017.00047
Tedjakumala SR, Rouquette J, Boizeau ML, Mesce KA, Hotier L, Massou I et al. A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain. Frontiers in Systems Neuroscience. 2017 Jul 10;11. 47. Available from, DOI: 10.3389/fnsys.2017.00047

Tedjakumala, Stevanus R.; Rouquette, Jacques; Boizeau, Marie Laure; Mesce, Karen A.; Hotier, Lucie; Massou, Isabelle; Giurfa, Martin / A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain.

In: Frontiers in Systems Neuroscience, Vol. 11, 47, 10.07.2017.

Research output: Contribution to journalArticle

@article{ed72da402e0546728afc7afe608b7c9f,
title = "A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain",
abstract = "Dopamine (DA) plays a fundamental role in insect behavior as it acts both as a general modulator of behavior and as a value system in associative learning where it mediates the reinforcing properties of unconditioned stimuli (US). Here we aimed at characterizing the dopaminergic neurons in the central nervous system of the honey bee, an insect that serves as an established model for the study of learning and memory. We used tyrosine hydroxylase (TH) immunoreactivity (ir) to ensure that the neurons detected synthesize DA endogenously. We found three main dopaminergic clusters, C1–C3, which had been previously described; the C1 cluster is located in a small region adjacent to the esophagus (ES) and the antennal lobe (AL); the C2 cluster is situated above the C1 cluster, between the AL and the vertical lobe (VL) of the mushroom body (MB); the C3 cluster is located below the calyces (CA) of the MB. In addition, we found a novel dopaminergic cluster, C4, located above the dorsomedial border of the lobula, which innervates the visual neuropils of the bee brain. Additional smaller processes and clusters were found and are described. The profuse dopaminergic innervation of the entire bee brain and the specific connectivity of DA neurons, with visual, olfactory and gustatory circuits, provide a foundation for a deeper understanding of how these sensory modules are modulated by DA, and the DA-dependent value-based associations that occur during associative learning.",
keywords = "Apis mellifera, Dopamine, Dopaminergic signaling, Neural circuits, Neural clusters",
author = "Tedjakumala, {Stevanus R.} and Jacques Rouquette and Boizeau, {Marie Laure} and Mesce, {Karen A.} and Lucie Hotier and Isabelle Massou and Martin Giurfa",
year = "2017",
month = "7",
doi = "10.3389/fnsys.2017.00047",
volume = "11",
journal = "Frontiers in Systems Neuroscience",
issn = "1662-5137",
publisher = "Frontiers Research Foundation",

}

TY - JOUR

T1 - A tyrosine-hydroxylase characterization of dopaminergic neurons in the honey bee brain

AU - Tedjakumala,Stevanus R.

AU - Rouquette,Jacques

AU - Boizeau,Marie Laure

AU - Mesce,Karen A.

AU - Hotier,Lucie

AU - Massou,Isabelle

AU - Giurfa,Martin

PY - 2017/7/10

Y1 - 2017/7/10

N2 - Dopamine (DA) plays a fundamental role in insect behavior as it acts both as a general modulator of behavior and as a value system in associative learning where it mediates the reinforcing properties of unconditioned stimuli (US). Here we aimed at characterizing the dopaminergic neurons in the central nervous system of the honey bee, an insect that serves as an established model for the study of learning and memory. We used tyrosine hydroxylase (TH) immunoreactivity (ir) to ensure that the neurons detected synthesize DA endogenously. We found three main dopaminergic clusters, C1–C3, which had been previously described; the C1 cluster is located in a small region adjacent to the esophagus (ES) and the antennal lobe (AL); the C2 cluster is situated above the C1 cluster, between the AL and the vertical lobe (VL) of the mushroom body (MB); the C3 cluster is located below the calyces (CA) of the MB. In addition, we found a novel dopaminergic cluster, C4, located above the dorsomedial border of the lobula, which innervates the visual neuropils of the bee brain. Additional smaller processes and clusters were found and are described. The profuse dopaminergic innervation of the entire bee brain and the specific connectivity of DA neurons, with visual, olfactory and gustatory circuits, provide a foundation for a deeper understanding of how these sensory modules are modulated by DA, and the DA-dependent value-based associations that occur during associative learning.

AB - Dopamine (DA) plays a fundamental role in insect behavior as it acts both as a general modulator of behavior and as a value system in associative learning where it mediates the reinforcing properties of unconditioned stimuli (US). Here we aimed at characterizing the dopaminergic neurons in the central nervous system of the honey bee, an insect that serves as an established model for the study of learning and memory. We used tyrosine hydroxylase (TH) immunoreactivity (ir) to ensure that the neurons detected synthesize DA endogenously. We found three main dopaminergic clusters, C1–C3, which had been previously described; the C1 cluster is located in a small region adjacent to the esophagus (ES) and the antennal lobe (AL); the C2 cluster is situated above the C1 cluster, between the AL and the vertical lobe (VL) of the mushroom body (MB); the C3 cluster is located below the calyces (CA) of the MB. In addition, we found a novel dopaminergic cluster, C4, located above the dorsomedial border of the lobula, which innervates the visual neuropils of the bee brain. Additional smaller processes and clusters were found and are described. The profuse dopaminergic innervation of the entire bee brain and the specific connectivity of DA neurons, with visual, olfactory and gustatory circuits, provide a foundation for a deeper understanding of how these sensory modules are modulated by DA, and the DA-dependent value-based associations that occur during associative learning.

KW - Apis mellifera

KW - Dopamine

KW - Dopaminergic signaling

KW - Neural circuits

KW - Neural clusters

UR - http://www.scopus.com/inward/record.url?scp=85026636158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026636158&partnerID=8YFLogxK

U2 - 10.3389/fnsys.2017.00047

DO - 10.3389/fnsys.2017.00047

M3 - Article

VL - 11

JO - Frontiers in Systems Neuroscience

T2 - Frontiers in Systems Neuroscience

JF - Frontiers in Systems Neuroscience

SN - 1662-5137

M1 - 47

ER -