A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus.

D. L. Hartley, M. K. Speedie

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalThe Biochemical journal
Volume220
Issue number1
DOIs
StatePublished - May 15 1984

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