Abstract
The maize Activator (Ac)/Dissociation (Ds) transposable element system has been used in a variety of plants for insertional mutagenesis. Ac/Ds elements can also generate genome rearrangements via alternative transposition reactions which involve the termini of closely linked transposons. Here, we introduced a transgene containing reverse-oriented Ac/Ds termini together with an Ac transposase gene into rice (Oryza sativa ssp. japonica cv. Nipponbare). Among the transgenic progeny, we identified and characterized 25 independent genome rearrangements at three different chromosomal loci. The rearrangements include chromosomal deletions and inversions and one translocation. Most of the deletions occurred within the T-DNA region, but two cases showed the loss of 72 kilobase pairs (kb) and 79 kb of rice genomic DNA flanking the transgene. In addition to deletions, we obtained chromosomal inversions ranging in size from less than 10 kb (within the transgene DNA) to over 1 million base pairs (Mb). For 11 inversions, we cloned and sequenced both inversion breakpoints; in all 11 cases, the inversion junctions contained the typical 8 base pairs (bp) Ac/Ds target site duplications, confirming their origin as transposition products. Together, our results indicate that alternative Ac/Ds transposition can be an efficient tool for functional genomics and chromosomal manipulation in rice.
Original language | English (US) |
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Pages (from-to) | 1449-1462 |
Number of pages | 14 |
Journal | Theoretical and Applied Genetics |
Volume | 125 |
Issue number | 7 |
DOIs | |
State | Published - Nov 2012 |
Externally published | Yes |
Bibliographical note
Funding Information:We gratefully acknowledge Venkatesan Sundaresan for providing the pSQ5 plasmid. We thank Bing Yang for providing Agrobacterium EHA105 strain and for suggestions on rice plant culture. We thank the ISU Plant Transformation Facility for rice transformation, the laboratories of Jo Ann Powell-Coffman and Jeff Essner for assistance with fluorescence microscopy, and Erik Vollbrecht for comments and suggestions. This work was supported by the National Science Foundation [0110170 and 0450243 to TAP, and DBI 0423898 to JB].