A toolbox of recombinant lipases for industrial applications

Claudia Schmidt-Dannert, Jürgen Pleiss, Rolf D. Schmid

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We created a toolbox of recombinant, microbial lipases, which allows us in combination with a lipase database to choose among the overexpressed lipases the most appropriate for a specific application and to improve it further via mutagenesis. By systematic comparison of geometry and properties of the scissile fatty acid binding site of five representative lipases of each family of structurally homologous lipases, three subgroups can be defined. Hence, efficient expression systems for the functional production of large amounts of microbial lipases, representing different lipase subgroups, were developed. In particular, recombinant lipases from Bacillus thermocatenulatus and Pseudomonas cepacia were functionally overexpressed in E. coli. The lipase genes from Geotrichum candidum CMICC 335426 and Rhizopus oryzae were overexpressed in Pichia pastoris. Due to an unusual codon usage that prevents heterologous expression, the LIP1 gene (1647 nt) of Candida rugosa was completely synthesized and overexpressed in Pichia pastoris.

Original languageEnglish (US)
Pages (from-to)14-22
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume864
DOIs
StatePublished - Jan 1 1998

Fingerprint Dive into the research topics of 'A toolbox of recombinant lipases for industrial applications'. Together they form a unique fingerprint.

Cite this