A thermostable transketolase evolved for aliphatic aldehyde acceptors

Dong Yi; Thangavelu Saravanan; Titu Devamani; Franck Charmantray; Laurence Hecquet; Wolf Dieter Fessner

doi:10.1039/c4cc08436e

A thermostable transketolase evolved for aliphatic aldehyde acceptors

Dong Yi
, Thangavelu Saravanan
, Titu Devamani
, Franck Charmantray
, Laurence Hecquet
, Wolf Dieter Fessner

Biotechnology Institute

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.

Original language	English (US)
Pages (from-to)	480-483
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	3
DOIs	https://doi.org/10.1039/c4cc08436e
State	Published - Jan 11 2015

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© The Royal Society of Chemistry 2015.

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10.1039/c4cc08436e

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abstract = "Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95\% ee) in the asymmetric carboligation step.",

author = "Dong Yi and Thangavelu Saravanan and Titu Devamani and Franck Charmantray and Laurence Hecquet and Fessner, \{Wolf Dieter\}",

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AU - Yi, Dong

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AU - Devamani, Titu

AU - Charmantray, Franck

AU - Hecquet, Laurence

AU - Fessner, Wolf Dieter

PY - 2015/1/11

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AB - Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.

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A thermostable transketolase evolved for aliphatic aldehyde acceptors

Abstract

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