A Sustained, Cytoplasmic Transgene Expression System Delivered by Cationic Liposomes

Xiang Gao, Daniel Jaffurs, Paul D. Robbins, Leaf Huang

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

A plasmid containing the reporter gene, chloramphenicol acetyltransferase (CAT), driven by the bacteriophage T7 promoter was co-delivered with purified T7 RNA polymerase by the DC-chol cationic liposomes into human embryonic kidney 293 cells to obtain a transient (2 days) CAT gene expression. To prolong the expression, a T7 autogene which contains the T7 RNA polymerase gene driven by the T7 promoter was included in the transfection complex as a self-amplifying regeneration mechanism for the polymerase. High level CAT gene expression was observed up to 5 days after transfection. This strong and sustained expression system should be useful in gene transfer experiments.

Original languageEnglish (US)
Pages (from-to)1201-1206
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number3
DOIs
StatePublished - May 15 1994
Externally publishedYes

Fingerprint Dive into the research topics of 'A Sustained, Cytoplasmic Transgene Expression System Delivered by Cationic Liposomes'. Together they form a unique fingerprint.

Cite this