A Survey of 6,300 Genomic Fragments for cis-Regulatory Activity in the Imaginal Discs of Drosophila melanogaster

Aurélie Jory, Carlos Estella, Matt W. Giorgianni, Matthew Slattery, Todd R. Laverty, Gerald M. Rubin, Richard S. Mann

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Over 6,000 fragments from the genome of Drosophila melanogaster were analyzed for their ability to drive expression of GAL4 reporter genes in the third-instar larval imaginal discs. About 1,200 reporter genes drove expression in the eye, antenna, leg, wing, haltere, or genital imaginal discs. The patterns ranged from large regions to individual cells. About 75% of the active fragments drove expression in multiple discs; 20% were expressed in ventral, but not dorsal, discs (legs, genital, and antenna), whereas ∼23% were expressed in dorsal but not ventral discs (wing, haltere, and eye). Several patterns, for example, within the leg chordotonal organ, appeared a surprisingly large number of times. Unbiased searches for DNA sequence motifs suggest candidate transcription factors that may regulate enhancers with shared activities. Together, these expression patterns provide a valuable resource to the community and offer a broad overview of how transcriptional regulatory information is distributed in the Drosophila genome

Original languageEnglish (US)
Pages (from-to)1014-1024
Number of pages11
JournalCell reports
Volume2
Issue number4
DOIs
StatePublished - Oct 25 2012

Bibliographical note

Funding Information:
We thank Marc Smith for help with the screen, Gary Struhl and Andrew Tomlinson for advice on disc annotations, Crystal Sullivan for organizing trips to JFRC, and Chris Doe for comments on the manuscript. This work was supported by the Janelia Farm Visitor Program and National Institutes of Health grants GM054510 and GM058575 awarded to R.S.M.

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