A study of cold cardioplegic myocardial protection in rats: An experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury

C. G.A. Mcgregor; J. Hannan; A. F. Smith; A. L. Muir; D. J. Wheatley

doi:10.1093/cvr/17.2.70

A study of cold cardioplegic myocardial protection in rats: An experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury

C. G.A. Mcgregor, J. Hannan, A. F. Smith, A. L. Muir, D. J. Wheatley

Research output: Contribution to journal › Review article › peer-review

6 Scopus citations

Abstract

Summary: This study compares the use of St Thomas' cardioplegia and cold saline cardioplegia at 4°C for protection against global myocardial ischaemia in a model of heterotopic cardiac transplantation in rats of isogeneic strain. The parameters of myocardial injury applied were the uptake of Technetium 99m pyrophosphate (Tc 99m Pyp) in the transplanted heart and the measurement of serum total creatine kinase (CK) and creatine kinase MB (CK:MB) isoenzyme activity. The findings indicate that St Thomas' cardioplegic solution confers improved myocardial protection compared with normal saline as judged by statistically significant differences between: (a) the uptake of Tc 99m Pyp between the two solutions at 30 min storage: and (b) the peak total serum creatine kinase enzyme activity at 90 min storage. Excellent correlation was present between the uptake of Tc 99m Pyp and peak total serum creatine kinase activity. Further application of this model is described.

Original language	English (US)
Pages (from-to)	70-74
Number of pages	5
Journal	Cardiovascular Research
Volume	17
Issue number	2
DOIs	https://doi.org/10.1093/cvr/17.2.70
State	Published - Feb 1983
Externally published	Yes

Bibliographical note

Funding Information:
The work was supported by a grant from the Scottish Hospital Endowment Research Trust.

Keywords

Cardioplegia
Enzymes
Myocardium (rat)
Tc 99m pyrophosphate

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10.1093/cvr/17.2.70

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title = "A study of cold cardioplegic myocardial protection in rats: An experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury",

abstract = "Summary: This study compares the use of St Thomas' cardioplegia and cold saline cardioplegia at 4°C for protection against global myocardial ischaemia in a model of heterotopic cardiac transplantation in rats of isogeneic strain. The parameters of myocardial injury applied were the uptake of Technetium 99m pyrophosphate (Tc 99m Pyp) in the transplanted heart and the measurement of serum total creatine kinase (CK) and creatine kinase MB (CK:MB) isoenzyme activity. The findings indicate that St Thomas' cardioplegic solution confers improved myocardial protection compared with normal saline as judged by statistically significant differences between: (a) the uptake of Tc 99m Pyp between the two solutions at 30 min storage: and (b) the peak total serum creatine kinase enzyme activity at 90 min storage. Excellent correlation was present between the uptake of Tc 99m Pyp and peak total serum creatine kinase activity. Further application of this model is described.",

keywords = "Cardioplegia, Enzymes, Myocardium (rat), Tc 99m pyrophosphate",

author = "Mcgregor, {C. G.A.} and J. Hannan and Smith, {A. F.} and Muir, {A. L.} and Wheatley, {D. J.}",

note = "Funding Information: The work was supported by a grant from the Scottish Hospital Endowment Research Trust.",

year = "1983",

month = feb,

doi = "10.1093/cvr/17.2.70",

language = "English (US)",

volume = "17",

pages = "70--74",

journal = "Cardiovascular Research",

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T1 - A study of cold cardioplegic myocardial protection in rats

T2 - An experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury

AU - Mcgregor, C. G.A.

AU - Hannan, J.

AU - Smith, A. F.

AU - Muir, A. L.

AU - Wheatley, D. J.

N1 - Funding Information: The work was supported by a grant from the Scottish Hospital Endowment Research Trust.

PY - 1983/2

Y1 - 1983/2

N2 - Summary: This study compares the use of St Thomas' cardioplegia and cold saline cardioplegia at 4°C for protection against global myocardial ischaemia in a model of heterotopic cardiac transplantation in rats of isogeneic strain. The parameters of myocardial injury applied were the uptake of Technetium 99m pyrophosphate (Tc 99m Pyp) in the transplanted heart and the measurement of serum total creatine kinase (CK) and creatine kinase MB (CK:MB) isoenzyme activity. The findings indicate that St Thomas' cardioplegic solution confers improved myocardial protection compared with normal saline as judged by statistically significant differences between: (a) the uptake of Tc 99m Pyp between the two solutions at 30 min storage: and (b) the peak total serum creatine kinase enzyme activity at 90 min storage. Excellent correlation was present between the uptake of Tc 99m Pyp and peak total serum creatine kinase activity. Further application of this model is described.

AB - Summary: This study compares the use of St Thomas' cardioplegia and cold saline cardioplegia at 4°C for protection against global myocardial ischaemia in a model of heterotopic cardiac transplantation in rats of isogeneic strain. The parameters of myocardial injury applied were the uptake of Technetium 99m pyrophosphate (Tc 99m Pyp) in the transplanted heart and the measurement of serum total creatine kinase (CK) and creatine kinase MB (CK:MB) isoenzyme activity. The findings indicate that St Thomas' cardioplegic solution confers improved myocardial protection compared with normal saline as judged by statistically significant differences between: (a) the uptake of Tc 99m Pyp between the two solutions at 30 min storage: and (b) the peak total serum creatine kinase enzyme activity at 90 min storage. Excellent correlation was present between the uptake of Tc 99m Pyp and peak total serum creatine kinase activity. Further application of this model is described.

KW - Cardioplegia

KW - Enzymes

KW - Myocardium (rat)

KW - Tc 99m pyrophosphate

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ER -

A study of cold cardioplegic myocardial protection in rats: An experimental model using the uptake of technetium 99m pyrophosphate and enzyme activity as parameters of injury

Abstract

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Keywords

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