A study of cAMP binding proteins on intact and disrupted sperm cells using 8‐azidoadenosine 3′,5′‐cyclic monophoshate

Patrick K. Schoff, Ian T. Forrester, Boyd E. Haley, Robert W. Atherton

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Abstract

The photoaffinity probe (32P)8‐N3 cAMP was used to label the cAMP binding proteins in washed ejaculated human sperm. Three saturable binding proteins were photolabeled in both intact and disrupted cells with apparent molecular weights of 55,000, 49,000 and 40,000 daltons corresponding to the regulatory subunits of type II and type I cAMP‐dependent protein kinase (cAMP‐PK) and to an endogenous proteolytic product of the regulatory subunits, respectively. Photoincorporation in the three proteins could be totally blocked by preincubating the cells with cAMP. Cell‐free seminal plasma was found to be free of detectable (12P) 8‐N3 cAMP‐binding proteins. The 8‐N3 cAMP was also effective in stimulating endogenous cAMP‐PK activity in intact and disrupted sperm. A substantial amount of (32P) 8‐N3 cAMP binding to types I and II regulatory subunits and cAMP‐PK activity was detected on washed intact cells, intact cells. Intact cell bound 1.80 pmol of (32P) 8‐N3 cAMP/mg protein and had cAMP‐PK activity of 824 units/108 cells. Disrupted cells bound 3.95 pmol (32P) 8‐N3 cAMP mg protein and had a cAMP‐PK activity of 2,206 units/108 cells. The data presented support the concept of two classes of cAMP receptors being differentially available to externally added (32P) 8‐N3 cAMP and proteases. Cellular membrane integrity and membrane sidedness are discussed as possible explanations for the observation reported.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalJournal of Cellular Biochemistry
Volume19
Issue number1
DOIs
StatePublished - 1982

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Keywords

  • ejaculated sperm
  • membrane sidedness
  • photoaffinity
  • regulatory subunits

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