A strategy for labeling tumor boundaries

Jeanne P. Haushalter; Xudong Xiao; Khalid Amin; Zishan Haroon; Wan Ru Chao; Gregory W. Faris

doi:10.1364/fio.2006.jtha4

A strategy for labeling tumor boundaries

Jeanne P. Haushalter
, Xudong Xiao
, Khalid Amin
, Zishan Haroon
, Wan Ru Chao
, Gregory W. Faris

Laboratory Medicine and Pathology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We describe a method for labeling tumor boundaries using an enzyme active in the tumor boundary to crosslink a fluorescent-labeled substrate into the boundary. Preliminary in vitro assays are described.

Original language	English (US)
Title of host publication	Laser Science, LS 2006
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Electronic)	9781557528209
ISBN (Print)	1557528187, 9781557528186
DOIs	https://doi.org/10.1364/fio.2006.jtha4
State	Published - 2006
Event	Laser Science, LS 2006 - Rochester, NY, United States Duration: Oct 10 2006 → Oct 10 2006

Publication series

Name	Optics InfoBase Conference Papers

Other

Other	Laser Science, LS 2006
Country/Territory	United States
City	Rochester, NY
Period	10/10/06 → 10/10/06

Bibliographical note

Funding Information:
We are studying a method for specific labeling of tumor boundaries using infrared fluorescence. This method is based on the action of the enzyme tissue transglutaminase, an enzyme is involved in the wound-healing response [1], where it helps stabilize the extracellular matrix. The enzyme has also been shown to be involved in neovasculature and extracellular matrix at the boundary between tumors and normal tissue [2,3]. We plan to use the tissue transglutaminase activity to crosslink substrates conjugated to fluorescent infrared dyes to label the tumor boundary. Fig. 1 shows results of an in vitro assay for tissue transglutaminase activity based on scratch injuries of confluent EA-hy296 cells. This research was supported by funds from DOD Breast Cancer Research Program, Grant Number W81XWH-05-1-0386 and the National Cancer Institute, Grant Number 1R21 CA107285

Publisher Copyright:
© OSA/FiO/LS/OPE 2006.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1364/fio.2006.jtha4

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title = "A strategy for labeling tumor boundaries",

abstract = "We describe a method for labeling tumor boundaries using an enzyme active in the tumor boundary to crosslink a fluorescent-labeled substrate into the boundary. Preliminary in vitro assays are described.",

author = "Haushalter, \{Jeanne P.\} and Xudong Xiao and Khalid Amin and Zishan Haroon and Chao, \{Wan Ru\} and Faris, \{Gregory W.\}",

note = "Funding Information: We are studying a method for specific labeling of tumor boundaries using infrared fluorescence. This method is based on the action of the enzyme tissue transglutaminase, an enzyme is involved in the wound-healing response [1], where it helps stabilize the extracellular matrix. The enzyme has also been shown to be involved in neovasculature and extracellular matrix at the boundary between tumors and normal tissue [2,3]. We plan to use the tissue transglutaminase activity to crosslink substrates conjugated to fluorescent infrared dyes to label the tumor boundary. Fig. 1 shows results of an in vitro assay for tissue transglutaminase activity based on scratch injuries of confluent EA-hy296 cells. This research was supported by funds from DOD Breast Cancer Research Program, Grant Number W81XWH-05-1-0386 and the National Cancer Institute, Grant Number 1R21 CA107285 Publisher Copyright: {\textcopyright} OSA/FiO/LS/OPE 2006.; Laser Science, LS 2006 ; Conference date: 10-10-2006 Through 10-10-2006",

year = "2006",

doi = "10.1364/fio.2006.jtha4",

language = "English (US)",

isbn = "1557528187",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "Laser Science, LS 2006",

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TY - GEN

T1 - A strategy for labeling tumor boundaries

AU - Haushalter, Jeanne P.

AU - Xiao, Xudong

AU - Amin, Khalid

AU - Haroon, Zishan

AU - Chao, Wan Ru

AU - Faris, Gregory W.

N1 - Funding Information: We are studying a method for specific labeling of tumor boundaries using infrared fluorescence. This method is based on the action of the enzyme tissue transglutaminase, an enzyme is involved in the wound-healing response [1], where it helps stabilize the extracellular matrix. The enzyme has also been shown to be involved in neovasculature and extracellular matrix at the boundary between tumors and normal tissue [2,3]. We plan to use the tissue transglutaminase activity to crosslink substrates conjugated to fluorescent infrared dyes to label the tumor boundary. Fig. 1 shows results of an in vitro assay for tissue transglutaminase activity based on scratch injuries of confluent EA-hy296 cells. This research was supported by funds from DOD Breast Cancer Research Program, Grant Number W81XWH-05-1-0386 and the National Cancer Institute, Grant Number 1R21 CA107285 Publisher Copyright: © OSA/FiO/LS/OPE 2006.

PY - 2006

Y1 - 2006

N2 - We describe a method for labeling tumor boundaries using an enzyme active in the tumor boundary to crosslink a fluorescent-labeled substrate into the boundary. Preliminary in vitro assays are described.

AB - We describe a method for labeling tumor boundaries using an enzyme active in the tumor boundary to crosslink a fluorescent-labeled substrate into the boundary. Preliminary in vitro assays are described.

UR - https://www.scopus.com/pages/publications/85085403155

UR - https://www.scopus.com/pages/publications/85085403155#tab=citedBy

U2 - 10.1364/fio.2006.jtha4

DO - 10.1364/fio.2006.jtha4

M3 - Conference contribution

AN - SCOPUS:85085403155

SN - 1557528187

SN - 9781557528186

T3 - Optics InfoBase Conference Papers

BT - Laser Science, LS 2006

PB - Optica Publishing Group (formerly OSA)

T2 - Laser Science, LS 2006

Y2 - 10 October 2006 through 10 October 2006

ER -

A strategy for labeling tumor boundaries

Abstract

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Bibliographical note

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