A stochastic analysis of actin polymerization in the presence of twinfilin and gelsolin

Anastasios Matzavinos, Hans G Othmer

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We develop an efficient stochastic simulation algorithm for analyzing actin filament growth and decay in the presence of various actin-binding proteins. The evolution of nucleotide profiles of filaments can be tracked and the resulting feedback to actin-binding proteins is incorporated. The computational efficiency of the new method enables us to focus on experimentally realistic problems, and as one example we use it to analyze the experimental data of Helfer et al. [(2006). Mammalian twinfilin sequesters ADP-G-actin and caps filament barbed ends: implications in motility. EMBO J. 25, 1184-1195] on the capping and G-actin sequestering activity of twinfilin. We show that the binding specificity of twinfilin for ADP-G-actin is crucial for the observed biphasic evolution of the filament length distribution in the presence of twinfilin, and we demonstrate that twinfilin can be an essential part of the molecular machinery for regulating filament lengths after a short burst of polymerization. Significantly, our simulations indicate that the pyrenyl-actin fluorescence experiments would fail to report the emergence of large filaments under certain experimental conditions.

Original languageEnglish (US)
Pages (from-to)723-736
Number of pages14
JournalJournal of Theoretical Biology
Volume249
Issue number4
DOIs
StatePublished - Dec 21 2007

Keywords

  • Actin
  • Capping protein
  • Gelsolin
  • Polymerization
  • Stochastic simulation algorithm
  • Twinfilin

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