A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications

Yi Wang; Shainnel O. Eans; Heather M. Stacy; Sreekanth C. Narayanapillai; Abhisheak Sharma; Naomi Fujioka; Linda Haddad; Jay McLaughlin; Bonnie A. Avery; Chengguo Xing

doi:10.1371/journal.pone.0197940

A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications

Yi Wang, Shainnel O. Eans, Heather M. Stacy, Sreekanth C. Narayanapillai, Abhisheak Sharma, Naomi Fujioka, Linda Haddad, Jay McLaughlin, Bonnie A. Avery, Chengguo Xing

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) with excellent selectivity and specificity. The method has been validated for different matrices following the Food and Drug Administration guidance of analytical procedures and methods validation. The scope of this method has been demonstrated by quantifying these kavalactones in two kava products, characterizing their tissue distribution and pharmacokinetics in mice, and detecting their presence in human urines and plasmas upon kava intake. As expected, the abundances of these kavalactones differed significantly in kava products. All of them exhibited a large volume of distribution with extensive tissue affinity and adequate mean residence time (MRT) in mice. This method also successfully quantified these kavalactones in human body fluids upon kava consumption at the recommended human dose. This UPLC-MS/MS method therefore can be used to characterize kava products and its pharmacokinetics in animals and in humans.

Original language	English (US)
Article number	e0197940
Journal	PloS one
Volume	13
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0197940
State	Published - May 2018

Bibliographical note

Funding Information:
National Institute of Health R01CA193278 (CX), Masonic Cancer Center University of Minnesota (NF and CX), the Start-up fund from University of Florida College of Pharmacy and Department of Medicinal Chemistry (JPM and CX), and the COP PROSPER Award (CX). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2018 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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10.1371/journal.pone.0197940

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993114

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title = "A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications",

abstract = "Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) with excellent selectivity and specificity. The method has been validated for different matrices following the Food and Drug Administration guidance of analytical procedures and methods validation. The scope of this method has been demonstrated by quantifying these kavalactones in two kava products, characterizing their tissue distribution and pharmacokinetics in mice, and detecting their presence in human urines and plasmas upon kava intake. As expected, the abundances of these kavalactones differed significantly in kava products. All of them exhibited a large volume of distribution with extensive tissue affinity and adequate mean residence time (MRT) in mice. This method also successfully quantified these kavalactones in human body fluids upon kava consumption at the recommended human dose. This UPLC-MS/MS method therefore can be used to characterize kava products and its pharmacokinetics in animals and in humans.",

author = "Yi Wang and Eans, {Shainnel O.} and Stacy, {Heather M.} and Narayanapillai, {Sreekanth C.} and Abhisheak Sharma and Naomi Fujioka and Linda Haddad and Jay McLaughlin and Avery, {Bonnie A.} and Chengguo Xing",

note = "Funding Information: National Institute of Health R01CA193278 (CX), Masonic Cancer Center University of Minnesota (NF and CX), the Start-up fund from University of Florida College of Pharmacy and Department of Medicinal Chemistry (JPM and CX), and the COP PROSPER Award (CX). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2018 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

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AU - Eans, Shainnel O.

AU - Stacy, Heather M.

AU - Narayanapillai, Sreekanth C.

AU - Sharma, Abhisheak

AU - Fujioka, Naomi

AU - Haddad, Linda

AU - McLaughlin, Jay

AU - Avery, Bonnie A.

AU - Xing, Chengguo

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PY - 2018/5

Y1 - 2018/5

N2 - Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) with excellent selectivity and specificity. The method has been validated for different matrices following the Food and Drug Administration guidance of analytical procedures and methods validation. The scope of this method has been demonstrated by quantifying these kavalactones in two kava products, characterizing their tissue distribution and pharmacokinetics in mice, and detecting their presence in human urines and plasmas upon kava intake. As expected, the abundances of these kavalactones differed significantly in kava products. All of them exhibited a large volume of distribution with extensive tissue affinity and adequate mean residence time (MRT) in mice. This method also successfully quantified these kavalactones in human body fluids upon kava consumption at the recommended human dose. This UPLC-MS/MS method therefore can be used to characterize kava products and its pharmacokinetics in animals and in humans.

AB - Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) with excellent selectivity and specificity. The method has been validated for different matrices following the Food and Drug Administration guidance of analytical procedures and methods validation. The scope of this method has been demonstrated by quantifying these kavalactones in two kava products, characterizing their tissue distribution and pharmacokinetics in mice, and detecting their presence in human urines and plasmas upon kava intake. As expected, the abundances of these kavalactones differed significantly in kava products. All of them exhibited a large volume of distribution with extensive tissue affinity and adequate mean residence time (MRT) in mice. This method also successfully quantified these kavalactones in human body fluids upon kava consumption at the recommended human dose. This UPLC-MS/MS method therefore can be used to characterize kava products and its pharmacokinetics in animals and in humans.

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A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications

Abstract

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