A spectrophotometric method for assay of tannase using rhodanine

Shweta Sharma, T. K. Bhat, R. K. Dawra

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalAnalytical Biochemistry
Volume279
Issue number1
DOIs
StatePublished - Mar 1 2000

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Rhodanine
Gallic Acid
Tannins
Hydrolases
Assays

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A spectrophotometric method for assay of tannase using rhodanine. / Sharma, Shweta; Bhat, T. K.; Dawra, R. K.

In: Analytical Biochemistry, Vol. 279, No. 1, 01.03.2000, p. 85-89.

Research output: Contribution to journalArticle

Sharma, Shweta ; Bhat, T. K. ; Dawra, R. K. / A spectrophotometric method for assay of tannase using rhodanine. In: Analytical Biochemistry. 2000 ; Vol. 279, No. 1. pp. 85-89.
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