A spectrophotometric method for assay of tannase using rhodanine

Shweta Sharma; T. K. Bhat; R. K. Dawra

doi:10.1006/abio.1999.4405

A spectrophotometric method for assay of tannase using rhodanine

Shweta Sharma, T. K. Bhat, R. K. Dawra

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Abstract

A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	85-89
Number of pages	5
Journal	Analytical Biochemistry
Volume	279
Issue number	1
DOIs	https://doi.org/10.1006/abio.1999.4405
State	Published - Mar 1 2000

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10.1006/abio.1999.4405

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abstract = "A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.",

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N2 - A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

AB - A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

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A spectrophotometric method for assay of tannase using rhodanine

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