A spectrophotometric method for assay of tannase using rhodanine

Shweta Sharma, T. K. Bhat, R. K. Dawra

Research output: Contribution to journalArticlepeer-review

223 Scopus citations


A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalAnalytical Biochemistry
Issue number1
StatePublished - Mar 1 2000


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