Abstract
Caenorhabditis elegans is a useful organism for testing chemical effects on physiology. Whole organism small molecule screens offer significant advantages for identifying biologically active chemical structures that can modify complex phenotypes such as lifespan. Described here is a simple protocol for producing hundreds of 96-well culture plates with fairly consistent numbers of C. elegans in each well. Next, we specified how to use these cultures to screen thousands of chemicals for effects on the lifespan of the nematode C. elegans. This protocol makes use of temperature sensitive sterile strains, agar plate conditions, and simple animal handling to facilitate the rapid and high throughput production of synchronized animal cultures for screening.
| Original language | English (US) |
|---|---|
| Article number | e56892 |
| Journal | Journal of Visualized Experiments |
| Volume | 2018 |
| Issue number | 133 |
| DOIs | |
| State | Published - Mar 20 2018 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank Dipa Bhaumik, Aaron Miller and Bob Hughes for technical assistance. Strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We are grateful to Georgia Woods and members of the Lithgow, Kapahi and Melov Labs for useful discussions. M.L. was supported by National Institutes of Health (NIH) Training Grant T32 AG000266 and an Ellison Medical Foundation/American Federation of Aging Research Post-Doctoral Fellowship. This work was supported by a grant from BioAge Labs, a Larry L. Hillblom Foundation grant, as well as National Institutes of Health grants UL1024917, supporting the Interdisciplinary Research Consortium on Geroscience, and 1R01AG029631-01A1 to G.J.L.
Publisher Copyright:
© 2018 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
Keywords
- Aging
- Biochemistry
- Caenorhabditis
- Chemical biology
- High throughput
- Issue 133
- Lifespan
- Screening