A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Theodore R. Oegema; Laurel B. Deloria; Michelle M. Fedewa; John C. Bischof; Jack L. Lewis

doi:10.1006/cryo.2000.2253

A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Theodore R. Oegema
, Laurel B. Deloria
, Michelle M. Fedewa
, John C. Bischof
, Jack L. Lewis

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me₂SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	370-375
Number of pages	6
Journal	Cryobiology
Volume	40
Issue number	4
DOIs	https://doi.org/10.1006/cryo.2000.2253
State	Published - 2000

Bibliographical note

Funding Information:
This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.

Keywords

Biology
Biomechanics
Cartilage
Cryoprotection

Publisher link

10.1006/cryo.2000.2253

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title = "A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog",

abstract = "A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90\% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.",

keywords = "Biology, Biomechanics, Cartilage, Cryoprotection",

author = "Oegema, \{Theodore R.\} and Deloria, \{Laurel B.\} and Fedewa, \{Michelle M.\} and Bischof, \{John C.\} and Lewis, \{Jack L.\}",

note = "Funding Information: This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.",

year = "2000",

doi = "10.1006/cryo.2000.2253",

language = "English (US)",

volume = "40",

pages = "370--375",

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T1 - A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

AU - Oegema, Theodore R.

AU - Deloria, Laurel B.

AU - Fedewa, Michelle M.

AU - Bischof, John C.

AU - Lewis, Jack L.

N1 - Funding Information: This work was supported by the SOTA-TEC Fund of the Blandin Foundation and The Catherine Mills Davis Endowment to the Bioengineering Program of the Department of Orthopaedic Surgery.

PY - 2000

Y1 - 2000

N2 - A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

AB - A method for cryopreserving a 100-μm-thick sheet of tissue produced by cultured rabbit chondrocytes has been developed. The method maintains cell viability and avoids tissue fracture and degradation of mechanical properties. A slow-freeze, fast-thaw procedure with 2 M Me2SO as the cryoprotectant resulted in no tissue fracture and approximately 90% viable cells after storage in culture flasks at -80°C. The cells in the retrieved tissue remained responsive to IL-1β, and tensile and fracture toughness properties of the tissue were not degraded by cryopreservation. (C) 2000 Academic Press.

KW - Biology

KW - Biomechanics

KW - Cartilage

KW - Cryoprotection

UR - https://www.scopus.com/pages/publications/0033849859

UR - https://www.scopus.com/pages/publications/0033849859#tab=citedBy

U2 - 10.1006/cryo.2000.2253

DO - 10.1006/cryo.2000.2253

M3 - Article

C2 - 10924268

AN - SCOPUS:0033849859

SN - 0011-2240

VL - 40

SP - 370

EP - 375

JO - Cryobiology

JF - Cryobiology

IS - 4

ER -

A simple cryopreservation method for the maintenance of cell viability and mechanical integrity of a cultured cartilage analog

Abstract

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