A Simple and Straightforward Approach for Generating Small, Stable, Homogeneous, Unilamellar 1-Palmitoyl 2-Oleoyl Phosphatidylcholine (POPC) Bilayer Vesicles

Yong-Guang Gao, Le Thi My Le, Amer Alam, Rhoderick E. Brown

Research output: Contribution to journalArticlepeer-review

Abstract

Various methods have been developed to generate phosphoglyceride liposomes. Approaches resulting in homogeneous populations of unilamellar bilayer vesicles are generally preferred to mimic various cell membrane situations, as well as to optimize aqueous solute trapping efficiency using the least amount of lipid for biotechnological purposes. Most are time-consuming, often tedious, or require specialized equipment, and produce vesicles with limited shelf-life at room temperature or in cold storage. Herein, we describe a straightforward approach that avoids the preceding complications and streamlines the construction of unilamellar bilayer vesicles from 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC)/dihexanoyl phosphatidylcholine (DHPC) bicelle mixtures at room temperature. The resulting vesicles are small (32-36 nm diameter), unilamellar, bilayer vesicles that are homogeneous, stable, and resistant to freeze-thaw alterations.

Original languageEnglish (US)
Article numbere4271
JournalBio-protocol
Volume11
Issue number24
DOIs
StatePublished - Dec 20 2021

Bibliographical note

Funding Information:
We are grateful for support from the National Institutes of Health via HL125353 and GM45928 and the Hormel Foundation. The approach detailed here was initially summarized in Gao et al. (2020). Anal Chem 92(4): 3417-3425.

Publisher Copyright:
© 2021 The Authors.

Keywords

  • 1-palmitoyl-2-oleoyl phosphatidylcholine
  • Bicelle lipid mixtures
  • Dihexanoyl phosphatidylcholine
  • Dilution
  • Phospholipid unilamellar bilayer vesicle production
  • Spontaneous formation

PubMed: MeSH publication types

  • Journal Article

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