A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini

P. L. Rutledge; A. Saluja; U. Leli; M. L. Steer

doi:10.1097/00006676-198810000-00002

A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini

P. L. Rutledge, A. Saluja, U. Leli, M. L. Steer

Surgery

Research output: Contribution to journal › Article › peer-review

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Abstract

A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-fi.m nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.

Original language	English (US)
Pages (from-to)	508-511
Number of pages	4
Journal	Pancreas
Volume	3
Issue number	5
DOIs	https://doi.org/10.1097/00006676-198810000-00002
State	Published - Oct 1988

Keywords

Enzyme assay
Ion fluxes
Pancreas
Secretion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1097/00006676-198810000-00002

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abstract = "A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-fi.m nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.",

keywords = "Enzyme assay, Ion fluxes, Pancreas, Secretion",

author = "Rutledge, {P. L.} and A. Saluja and U. Leli and Steer, {M. L.}",

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T1 - A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini

AU - Rutledge, P. L.

AU - Saluja, A.

AU - Leli, U.

AU - Steer, M. L.

PY - 1988/10

Y1 - 1988/10

N2 - A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-fi.m nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.

AB - A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-fi.m nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.

KW - Enzyme assay

KW - Ion fluxes

KW - Pancreas

KW - Secretion

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JO - Pancreas

JF - Pancreas

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ER -

A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini

Abstract

Keywords

UN SDGs

Publisher link

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