A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding α-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth. Copyright (C) 2000.
|Original language||English (US)|
|Number of pages||11|
|Journal||Journal of Microbiological Methods|
|State||Published - Jan 2000|
Bibliographical noteFunding Information:
The authors would like to thank William F. Liljemark and Cynthia G. Bloomquist, Department of Diagnostic and Surgical Sciences, University of Minnesota, for their technical advice and scientific insights. This work was supported by the 3M Dental Materials Center at the University of Minnesota.
Copyright 2007 Elsevier B.V., All rights reserved.
- Growth initiation
- Reporter gene fusion
- Streptococcus gordonii
- rRNA promoter