A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions

Arvind Natarajan; Mark Everard; Dawn Manias; Friedrich Srienc; Gary Dunny

doi:10.1016/S0167-7012(99)00105-0

A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions

Arvind Natarajan, Mark Everard, Dawn Manias, Friedrich Srienc, Gary Dunny

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding α-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth. Copyright (C) 2000.

Original language	English (US)
Pages (from-to)	133-143
Number of pages	11
Journal	Journal of Microbiological Methods
Volume	39
Issue number	2
DOIs	https://doi.org/10.1016/S0167-7012(99)00105-0
State	Published - Jan 2000

Bibliographical note

Funding Information:
The authors would like to thank William F. Liljemark and Cynthia G. Bloomquist, Department of Diagnostic and Surgical Sciences, University of Minnesota, for their technical advice and scientific insights. This work was supported by the 3M Dental Materials Center at the University of Minnesota.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

Growth initiation
Reporter gene fusion
Streptococcus gordonii
rRNA promoter

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10.1016/S0167-7012(99)00105-0

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title = "A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions",

abstract = "A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding α-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth. Copyright (C) 2000.",

keywords = "Growth initiation, Reporter gene fusion, Streptococcus gordonii, rRNA promoter",

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note = "Funding Information: The authors would like to thank William F. Liljemark and Cynthia G. Bloomquist, Department of Diagnostic and Surgical Sciences, University of Minnesota, for their technical advice and scientific insights. This work was supported by the 3M Dental Materials Center at the University of Minnesota. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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AU - Everard, Mark

AU - Manias, Dawn

AU - Srienc, Friedrich

AU - Dunny, Gary

N1 - Funding Information: The authors would like to thank William F. Liljemark and Cynthia G. Bloomquist, Department of Diagnostic and Surgical Sciences, University of Minnesota, for their technical advice and scientific insights. This work was supported by the 3M Dental Materials Center at the University of Minnesota. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

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N2 - A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding α-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth. Copyright (C) 2000.

AB - A system for studying the early growth response of Streptococcus gordonii to environmental stimuli has been developed. A reporter gene, encoding α-amylase, has been integrated into an rRNA operon to monitor changes in cellular physiology associated with the initiation of growth. Two such strains with single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to growth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rrn operons containing the reporter gene suggests that they are differentially expressed over the course of growth. Copyright (C) 2000.

KW - Growth initiation

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KW - Streptococcus gordonii

KW - rRNA promoter

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A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions

Abstract

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Keywords

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