A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi; Leah N. Frautschy; Frank B. Cerra; Wei-Shou Hu

doi:10.1007/BF00157378

A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi
, Leah N. Frautschy
, Frank B. Cerra
, Wei-Shou Hu

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 10⁶ cells. An OUR as low as 0.02 μmol/h can be accurately measured.

Original language	English (US)
Pages (from-to)	99-104
Number of pages	6
Journal	Biotechnology Techniques
Volume	7
Issue number	2
DOIs	https://doi.org/10.1007/BF00157378
State	Published - Feb 1993

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@article{35a2737a1d95469182a4e11322c71120,

title = "A sensitive measurement of oxygen uptake rate using a optochemical sensor",

abstract = "The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.",

author = "Chi, \{Chung Ming\} and Frautschy, \{Leah N.\} and Cerra, \{Frank B.\} and Wei-Shou Hu",

year = "1993",

month = feb,

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language = "English (US)",

volume = "7",

pages = "99--104",

journal = "Biotechnology Techniques",

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AU - Chi, Chung Ming

AU - Frautschy, Leah N.

AU - Cerra, Frank B.

AU - Hu, Wei-Shou

PY - 1993/2

Y1 - 1993/2

N2 - The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

AB - The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

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UR - https://www.scopus.com/pages/publications/34250079377#tab=citedBy

U2 - 10.1007/BF00157378

DO - 10.1007/BF00157378

M3 - Article

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SN - 0951-208X

VL - 7

SP - 99

EP - 104

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

A sensitive measurement of oxygen uptake rate using a optochemical sensor

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