A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi, Leah N. Frautschy, Frank B. Cerra, Wei-Shou Hu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalBiotechnology Techniques
Volume7
Issue number2
DOIs
StatePublished - Feb 1 1993

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Oxygen
Sensors
Daucus carota
Hybridomas
Oxygen Consumption
Sample Size
Electrodes
Embryonic Structures

Cite this

A sensitive measurement of oxygen uptake rate using a optochemical sensor. / Chi, Chung Ming; Frautschy, Leah N.; Cerra, Frank B.; Hu, Wei-Shou.

In: Biotechnology Techniques, Vol. 7, No. 2, 01.02.1993, p. 99-104.

Research output: Contribution to journalArticle

Chi, Chung Ming ; Frautschy, Leah N. ; Cerra, Frank B. ; Hu, Wei-Shou. / A sensitive measurement of oxygen uptake rate using a optochemical sensor. In: Biotechnology Techniques. 1993 ; Vol. 7, No. 2. pp. 99-104.
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