A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi, Leah N. Frautschy, Frank B. Cerra, Wei-Shou Hu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalBiotechnology Techniques
Volume7
Issue number2
DOIs
StatePublished - Feb 1 1993

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