A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi; Leah N. Frautschy; Frank B. Cerra; Wei-Shou Hu

doi:10.1007/BF00157378

A sensitive measurement of oxygen uptake rate using a optochemical sensor

Chung Ming Chi, Leah N. Frautschy, Frank B. Cerra, Wei-Shou Hu

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

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Abstract

The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 10⁶ cells. An OUR as low as 0.02 μmol/h can be accurately measured.

Original language	English (US)
Pages (from-to)	99-104
Number of pages	6
Journal	Biotechnology Techniques
Volume	7
Issue number	2
DOIs	https://doi.org/10.1007/BF00157378
State	Published - Feb 1993

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title = "A sensitive measurement of oxygen uptake rate using a optochemical sensor",

abstract = "The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.",

author = "Chi, {Chung Ming} and Frautschy, {Leah N.} and Cerra, {Frank B.} and Wei-Shou Hu",

year = "1993",

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AU - Frautschy, Leah N.

AU - Cerra, Frank B.

AU - Hu, Wei-Shou

PY - 1993/2

Y1 - 1993/2

N2 - The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

AB - The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with approximately 1000 cell clusters and of hybridoma with approximately 106 cells. An OUR as low as 0.02 μmol/h can be accurately measured.

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

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ER -

A sensitive measurement of oxygen uptake rate using a optochemical sensor

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