A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies

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Abstract

Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.

Original languageEnglish (US)
Pages (from-to)1071-1076
Number of pages6
JournalBiomedical Chromatography
Volume26
Issue number9
DOIs
StatePublished - Sep 1 2012

Keywords

  • Cyheptamide
  • Gas chromatography
  • Humans
  • Mass spectrometry
  • Topiramate

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