A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies

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Abstract

Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.

Original languageEnglish (US)
Pages (from-to)1071-1076
Number of pages6
JournalBiomedical Chromatography
Volume26
Issue number9
DOIs
StatePublished - Sep 1 2012

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Plasmas
Assays
Pharmacokinetics
Solid Phase Extraction
Migraine Disorders
Quality Control
Anticonvulsants
Quality control
Oral Administration
topiramate
Epilepsy

Keywords

  • Cyheptamide
  • Gas chromatography
  • Humans
  • Mass spectrometry
  • Topiramate

Cite this

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title = "A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies",
abstract = "Topiramate (Topamax{\circledR}) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3{\%} and the precision ({\%}CV) ranged from 1.0 to 5.3{\%} for both curves at all concentrations. The {\%}CV for quality controls was <7.6{\%}. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.",
keywords = "Cyheptamide, Gas chromatography, Humans, Mass spectrometry, Topiramate",
author = "Conway, {Jeannine M} and Birnbaum, {Angela K} and Marino, {Susan E} and Cloyd, {James C} and Remmel, {Rory P}",
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T1 - A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies

AU - Conway, Jeannine M

AU - Birnbaum, Angela K

AU - Marino, Susan E

AU - Cloyd, James C

AU - Remmel, Rory P

PY - 2012/9/1

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N2 - Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.

AB - Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 μg/mL and from 4 to 40 μg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.

KW - Cyheptamide

KW - Gas chromatography

KW - Humans

KW - Mass spectrometry

KW - Topiramate

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