A sensitive and selective liquid chromatography/tandem mass spectrometry method for determination of MLN8237 in human plasma

Emily Lipsitz, Ganesh Moorthy, Yael Mosse, Elizabeth Fox, Peter C. Adamson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We describe a selective and a highly sensitive high-performance liquid chromatography-electron spray ionization-collision induced dissociation-tandem mass spectrometry (HPLC-ESI-CID-MS/MS) assay for the Aurora A kinase inhibitor MLN8237 in human plasma. The intra-day precision based on the standard deviation of replicates of quality control samples ranged from 0.2 to 4% and with accuracy ranging from 96 to 102%. The inter-day precision ranged from 0.5 to 7% and the accuracy ranged from 93 to 105%. Stability studies showed that MLN8237 was stable both during the expected conditions for sample preparation and storage. The lower limit of quantification for MLN8237 was 5. ng/mL. The analytical method showed excellent sensitivity, precision, and accuracy. This method is robust and is being successfully employed in a Children's Oncology Group Phase 1 Consortium study of MLN8237 in children with cancer.

Original languageEnglish (US)
Pages (from-to)2369-2373
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume878
Issue number25
DOIs
StatePublished - Sep 1 2010

Keywords

  • Cancer
  • LC-ESI-CID-MS/MS
  • MLN8237
  • Pediatric
  • Plasma

Fingerprint Dive into the research topics of 'A sensitive and selective liquid chromatography/tandem mass spectrometry method for determination of MLN8237 in human plasma'. Together they form a unique fingerprint.

  • Cite this