A selective luminescent probe for the direct time-gated detection of adenosine triphosphate

Evan A. Weitz, Jennifer Y. Chang, Adam H. Rosenfield, Valerie C Pierre

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

A molecular probe for the luminescent detection of adenosine nucleotides is presented. The probe, Tb-DOTAm-Phen, readily distinguishes among the three adenosine nucleotides in buffered aqueous conditions at neutral pH, a requirement for the direct monitoring of enzymatic reactions converting adenosine triphosphate (ATP) to adenosine diphosphate or adenosine monophosphate. The probe is most efficient under millimolar concentrations of ATP which are relevant to intracellular conditions. Moreover, the long luminescence lifetime of the probe readily enables time-gating experiments.

Original languageEnglish (US)
Pages (from-to)16099-16102
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number39
DOIs
StatePublished - Oct 3 2012

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