Background: Autoantibody biomarkers are valuable tools used to diagnose and manage autoimmune diseases in dogs. However, prior publications have raised concerns over a lack of standardization and sufficient validation for the use of biomarkers in veterinary medicine. Objectives: Systematically compile primary research on autoantibody biomarkers for autoimmune disease in dogs, summarize their methodological features, and evaluate their quality; synthesize data supporting their use into a resource for veterinarians and researchers. Animals: Not used. Methods: Five indices were searched to identify studies for evaluation: PubMed, CAB Abstracts, Web of Science, Agricola, and SCOPUS. Two independent reviewers (AET and ELC) screened titles and abstracts for exclusion criteria followed by full-text review of remaining articles. Relevant studies were classified based on study objectives (biomarker, epitope, technique). Data on study characteristics and outcomes were synthesized in independent data tables for each classification. Results: Ninety-two studies qualified for final analysis (n = 49 biomarker, n = 9 epitope, and n = 34 technique studies). A high degree of heterogeneity in study characteristics and outcomes reporting was observed. Opportunities to strengthen future studies could include: (1) routine use of negative controls, (2) power analyses to inform sample sizes, (3) statistical analyses when appropriate, and (4) multiple detection techniques to confirm results. Conclusions: These findings provide a resource that will allow veterinary clinicians to efficiently evaluate the evidence supporting the use of autoantibody biomarkers, along with the varied methodological approaches used in their development.
Bibliographical noteFunding Information:
Funding provided by the United States Department of Health and Human Services, National Institutes of Health, for Amy E. Treeful with a NIH T32 Fellowship from the PharmacoNeuroImmunology (PNI) training grant (NIH/NIDA T32 DA007097). Funding provided by the United States Department of Health and Human Services, National Institutes of Health, for Emily L. Coffey from the Comparative Medicine and Pathology training grant (T32 OD010993‐15). Funding provided by the United States Department of Health and Human Services, National Institutes of Health Office of the Director, for Steven G. Friedenberg by a Special Emphasis Research Career Award, K01 OD027058.
© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
- immune-mediated diseases
- Autoimmune Diseases/diagnosis
- Dog Diseases/diagnosis
PubMed: MeSH publication types
- Journal Article