A role for MEIS1 in MLL-fusion gene leukemia

Ashish R. Kumar, Quanzhi Li, Wendy A. Hudson, Weili Chen, Thien Sam, Qing Yao, Erik A. Lund, Baolin Wu, Branden J. Kowal, John H. Kersey

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Leukemias with MLL rearrangements are characterized by high expression of the homeobox gene MEIS1. In these studies, we knocked down Meis1 expression by shRNA lentivirus transduction in murine Mll-AF9 leukemia cells. Meis1 knockdown resulted in decreased proliferation and survival of murine Mll-AF9 leukemia cells. We also observed reduced clonogenic capacity and increased monocytic differentiation. The establishment of leukemia in transplantation recipients was significantly delayed by Meis1 knockdown. Gene expression profiling of cells transduced with Meis1 shRNA showed reduced expression of genes associated with cell cycle entry and progression. shRNA-mediated knockdown of MEIS1 in human MLL-fusion gene leukemia cell lines resulted in reduced cell growth. These results show that MEIS1 expression is important for MLL-rearranged leukemias and suggest that MEIS1 promotes cell-cycle entry. Targeting MEIS1 may have therapeutic potential for treating leukemias expressing this transcription factor.

Original languageEnglish (US)
Pages (from-to)1756-1758
Number of pages3
JournalBlood
Volume113
Issue number8
DOIs
StatePublished - Feb 19 2009

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    Kumar, A. R., Li, Q., Hudson, W. A., Chen, W., Sam, T., Yao, Q., Lund, E. A., Wu, B., Kowal, B. J., & Kersey, J. H. (2009). A role for MEIS1 in MLL-fusion gene leukemia. Blood, 113(8), 1756-1758. https://doi.org/10.1182/blood-2008-06-163287