A rhesus macaque model of Asian-lineage Zika virus infection

Dawn M. Dudley; Matthew T Aliota; Emma L. Mohr; Andrea M. Weiler; Gabrielle Lehrer-Brey; Kim L. Weisgrau; Mariel S. Mohns; Meghan E. Breitbach; Mustafa N. Rasheed; Christina M. Newman; Dane D. Gellerup; Louise H. Moncla; Jennifer Post; Nancy Schultz-Darken; Michele L. Schotzko; Jennifer M. Hayes; Josh A. Eudailey; M. Anthony Moody; Sallie R. Permar; Shelby L. O'Connor; Eva G. Rakasz; Heather A. Simmons; Saverio Capuano; Thaddeus G. Golos; Jorge E. Osorio; Thomas C. Friedrich; David H. O'Connor

doi:10.1038/ncomms12204

A rhesus macaque model of Asian-lineage Zika virus infection

Dawn M. Dudley, Matthew T Aliota, Emma L. Mohr, Andrea M. Weiler, Gabrielle Lehrer-Brey, Kim L. Weisgrau, Mariel S. Mohns, Meghan E. Breitbach, Mustafa N. Rasheed, Christina M. Newman, Dane D. Gellerup, Louise H. Moncla, Jennifer Post, Nancy Schultz-Darken, Michele L. Schotzko, Jennifer M. Hayes, Josh A. Eudailey, M. Anthony Moody, Sallie R. Permar, Shelby L. O'ConnorEva G. Rakasz, Heather A. Simmons, Saverio Capuano, Thaddeus G. Golos, Jorge E. Osorio, Thomas C. Friedrich, David H. O'Connor

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

326 Scopus citations

Abstract

Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy.

Original language	English (US)
Article number	12204
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12204
State	Published - Jun 28 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1038/ncomms12204

Find It

Find It at U of M Libraries

Cite this

Dudley, D. M., Aliota, M. T., Mohr, E. L., Weiler, A. M., Lehrer-Brey, G., Weisgrau, K. L., Mohns, M. S., Breitbach, M. E., Rasheed, M. N., Newman, C. M., Gellerup, D. D., Moncla, L. H., Post, J., Schultz-Darken, N., Schotzko, M. L., Hayes, J. M., Eudailey, J. A., Anthony Moody, M., Permar, S. R., ... O'Connor, D. H. (2016). A rhesus macaque model of Asian-lineage Zika virus infection. Nature communications, 7, Article 12204. https://doi.org/10.1038/ncomms12204

Dudley, DM, Aliota, MT, Mohr, EL, Weiler, AM, Lehrer-Brey, G, Weisgrau, KL, Mohns, MS, Breitbach, ME, Rasheed, MN, Newman, CM, Gellerup, DD, Moncla, LH, Post, J, Schultz-Darken, N, Schotzko, ML, Hayes, JM, Eudailey, JA, Anthony Moody, M, Permar, SR, O'Connor, SL, Rakasz, EG, Simmons, HA, Capuano, S, Golos, TG, Osorio, JE, Friedrich, TC & O'Connor, DH 2016, 'A rhesus macaque model of Asian-lineage Zika virus infection', Nature communications, vol. 7, 12204. https://doi.org/10.1038/ncomms12204

@article{8453ada41918435caefede896543b8eb,

title = "A rhesus macaque model of Asian-lineage Zika virus infection",

abstract = "Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barr{\'e} syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy.",

author = "Dudley, {Dawn M.} and Aliota, {Matthew T} and Mohr, {Emma L.} and Weiler, {Andrea M.} and Gabrielle Lehrer-Brey and Weisgrau, {Kim L.} and Mohns, {Mariel S.} and Breitbach, {Meghan E.} and Rasheed, {Mustafa N.} and Newman, {Christina M.} and Gellerup, {Dane D.} and Moncla, {Louise H.} and Jennifer Post and Nancy Schultz-Darken and Schotzko, {Michele L.} and Hayes, {Jennifer M.} and Eudailey, {Josh A.} and {Anthony Moody}, M. and Permar, {Sallie R.} and O'Connor, {Shelby L.} and Rakasz, {Eva G.} and Simmons, {Heather A.} and Saverio Capuano and Golos, {Thaddeus G.} and Osorio, {Jorge E.} and Friedrich, {Thomas C.} and O'Connor, {David H.}",

year = "2016",

month = jun,

day = "28",

doi = "10.1038/ncomms12204",

language = "English (US)",

volume = "7",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Research",

}

TY - JOUR

T1 - A rhesus macaque model of Asian-lineage Zika virus infection

AU - Dudley, Dawn M.

AU - Aliota, Matthew T

AU - Mohr, Emma L.

AU - Weiler, Andrea M.

AU - Lehrer-Brey, Gabrielle

AU - Weisgrau, Kim L.

AU - Mohns, Mariel S.

AU - Breitbach, Meghan E.

AU - Rasheed, Mustafa N.

AU - Newman, Christina M.

AU - Gellerup, Dane D.

AU - Moncla, Louise H.

AU - Post, Jennifer

AU - Schultz-Darken, Nancy

AU - Schotzko, Michele L.

AU - Hayes, Jennifer M.

AU - Eudailey, Josh A.

AU - Anthony Moody, M.

AU - Permar, Sallie R.

AU - O'Connor, Shelby L.

AU - Rakasz, Eva G.

AU - Simmons, Heather A.

AU - Capuano, Saverio

AU - Golos, Thaddeus G.

AU - Osorio, Jorge E.

AU - Friedrich, Thomas C.

AU - O'Connor, David H.

PY - 2016/6/28

Y1 - 2016/6/28

N2 - Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy.

AB - Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=84976632397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976632397&partnerID=8YFLogxK

U2 - 10.1038/ncomms12204

DO - 10.1038/ncomms12204

M3 - Article

C2 - 27352279

AN - SCOPUS:84976632397

SN - 2041-1723

VL - 7

JO - Nature communications

JF - Nature communications

M1 - 12204

ER -

A rhesus macaque model of Asian-lineage Zika virus infection

Abstract

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this