To determine the seroprevalence of Anaplasma in Minnesota cattle, the database of the Veterinary Diagnostic Laboratory (VDL), University of Minnesota was searched over a 10 year period (2001 to July 2010). A total of 438,407 bovine serum samples were tested by complement fixation test (CFT) and competitive enzyme linked immunosorbent test (cELISA). The positive rate of seroprevalence by CFT was 2.4% out of 211,484 samples, with a 0.13% anticomplementary result. The VDL at the University of Minnesota started using cELISA after 2002, and CFT application was suspended as diagnostic test for anaplasmosis after 2005 due to its complexity. With cELISA, positive seroprevalence was 6.8% out of 226,923 samples. The results suggest that the cELISA test was more rapid and less complex than the CFT.
- cELISA test