A Recombinant Construct with the Silver Carp Somatotropin Gene Can Be Used for Production of Transgenic Carps

A recombinant construct ptMTa-scGH containing the silver carp growth hormone gene and a promoter-enhancer area of the rainbow trout metal lothionein A gene was microinjected in the cytoplasm of the fertilized carp eggs. It was shown that ptMTa-scGH in the ring form as a part of Bluescript plasmid vector induced specific developmental defects and death in the embryos. No such pathogenic effect was found when a linearized fragment tMTa-scGH was used. Sequences of the introduced genes were found in transgenic carps: fingerlings, one-and two-year old individuals. The results of testing of the studied construct in vivo suggest the possible use of linearized fragment tMTa-scGH in experiments aimed at the production of genetically modified carps.