Abstract
Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5' terminus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2519-2521 |
| Number of pages | 3 |
| Journal | Archives of Virology |
| Volume | 159 |
| Issue number | 9 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
Bibliographical note
Funding Information:This research was supported in part by the Ecuadorean Science and Technology Secretariat (SENESCYT) through the PROMETEO program. The authors also thank blackberry growers in Paute-Azuay and Tisaleo-Tungurahua for granting us access to their fields.