A rapid western blotting protocol for the Xenopus oocyte

Yaping Lin-Moshier, Jonathan S. Marchant

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca2+ signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in <1 h. Therefore, oocytes can be conveniently processed for western blotting to assess relative expression levels, even after a long day of Ca2+ imaging experiments.

Original languageEnglish (US)
Pages (from-to)262-265
Number of pages4
JournalCold Spring Harbor Protocols
Volume8
Issue number3
DOIs
StatePublished - Mar 2013

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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