A radiosensitive APC activity dissociates IL-2 secretion and activation-induced cell death by autoreactive T cell hybridomas

Shiv A. Prasad, Steven P. Fling, Dale S. Gregerson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

T cell hybridomas were generated from a LEW rat T cell line specific for the uveltogenic peptide bov-B1 of bovine retinal S-antigen. Using these autoreactive hybridomas, IL-2 production and activation-induced cell death (AICD) were dissociated as outcomes of activation. The self-reactive hybridomas secrete IL-2 and undergo AICD in response to antigen presented by non-irradiated syngeneic splenocytes, whereas antigen presentation by irradiated splenocytes induced only AICD. IL-2 production by a non-self reactive hybridoma was unaffected by irradiation of the APC. Pretreatment of the APC with phorbol ester or lipopolysaccharide and IL-4 protected their ability to induce IL-2 secretion after γ-irradiation. Although the co-stimulation-blocking reagent CTLA-4-Ig mimicked the effect of γ-irradiation by preventing IL-2 secretion but not AICD, B7 expression on the APC was not radiosensitive, nor did co-stimulation, provided 'in trans' with a B7-expressing thirdparty cell, reconstitute antigen-specific hybridoma IL-2 secretion in response to irradiated APC. In summary, the data show that IL-2 secretion and AICD of a self-reactive T cell hybridoma can be dissociated as consequences of TCR occupancy in the presence of a functional co-stimulatory signal. It is proposed that the signals producing these events are transduced through the TCR-CD3 complex alone and reflect the differential outcomes of high- and low-affinity interactions.

Original languageEnglish (US)
Pages (from-to)1787-1798
Number of pages12
JournalInternational Immunology
Volume7
Issue number11
DOIs
StatePublished - Nov 1 1995

Keywords

  • Autoimmunity
  • EAU
  • Radiosensitivity
  • S-antigen
  • Tolerance

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