A radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials

Paul C. Sadek; Peter W. Carr; Larry D. Bowers; Louis C. Haddad

doi:10.1016/0003-2697(86)90104-1

A radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials

Paul C. Sadek, Peter W. Carr, Larry D. Bowers, Louis C. Haddad

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amount and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.

Original language	English (US)
Pages (from-to)	359-371
Number of pages	13
Journal	Analytical Biochemistry
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(86)90104-1
State	Published - Mar 1986

Bibliographical note

Funding Information:
This work was supported in part by a grant from the National Science Foundation. The authors also thank the 3M Company (St. Paul. Minn.) for their gracious cooperation in providing instrumentation and counting facilities for this work.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

HPLC
adsorption
proteins

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10.1016/0003-2697(86)90104-1

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title = "A radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials",

abstract = "A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amount and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.",

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author = "Sadek, {Paul C.} and Carr, {Peter W.} and Bowers, {Larry D.} and Haddad, {Louis C.}",

note = "Funding Information: This work was supported in part by a grant from the National Science Foundation. The authors also thank the 3M Company (St. Paul. Minn.) for their gracious cooperation in providing instrumentation and counting facilities for this work. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

month = mar,

doi = "10.1016/0003-2697(86)90104-1",

language = "English (US)",

volume = "153",

pages = "359--371",

journal = "Analytical Biochemistry",

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T1 - A radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials

AU - Sadek, Paul C.

AU - Carr, Peter W.

AU - Bowers, Larry D.

AU - Haddad, Louis C.

N1 - Funding Information: This work was supported in part by a grant from the National Science Foundation. The authors also thank the 3M Company (St. Paul. Minn.) for their gracious cooperation in providing instrumentation and counting facilities for this work. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/3

Y1 - 1986/3

N2 - A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amount and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.

AB - A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amount and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.

KW - HPLC

KW - adsorption

KW - proteins

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JF - Analytical Biochemistry

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A radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials

Abstract

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