Abstract
A radial immunodiffusion enzyme assay (RIDEA) was developed for detection and quantitation of antibodies to equine herpes virus-1 (EHV-1) in horse sera. The detection and quantitation of EHV-1 antibody levels were based on the diameter of the radial diffusion zone of specific antibody in each serum sample reacting with EHV-1 antigen. The circular zone was made visible using peroxidase-conjugated rabbit anti-horse immunoglobulin G and a substrate containing hydrogen peroxide. The results of the RIDEA were compared with those of virus neutralization (VN) and enzyme-linked immunosorbent assay (ELISA) and found to be highly correlated. The relative sensitivity and specificity (percentage of agreement with VN test) were found to be 98.2 and 92.5%, respectively. Because the test procedure is relatively easy to perform, the RIDEA could be used as a field test to detect antibodies to EHV-1 in horses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 315-322 |
| Number of pages | 8 |
| Journal | Veterinary Microbiology |
| Volume | 17 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1988 |
Bibliographical note
Funding Information:This work was supported in part by Minnesota Racing Commission. We are grateful to Londa Kroone in the Veterinary Diagnostic Laboratory, University of Minnesota, for providing horse sera.