A rabbit monoclonal antibody against the antiviral and cancer genomic dna mutating enzyme apobec3b

William L. Brown, Emily K. Law, Prokopios Argyris, Michael A Carpenter, Rena Levin-Klein, Alison N. Ranum, Amy M Molan, Colleen L Forster, Brett D. Anderson, Lela Lackey, Reuben Harris

Research output: Contribution to journalArticlepeer-review

Abstract

The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B. One mAb, 5210-87-13, demonstrates utility in multiple applications, including ELISA, immunoblot, immunofluorescence microscopy, and immunohistochemistry. In head-to-head tests with commercial reagents, 5210-87-13 was the only rabbit monoclonal suitable for detecting native A3B and for immunohistochemical quantification of A3B in tumor tissues. This novel mAb has the potential to enable a wide range of fundamental and clinical studies on A3B in human biology and disease.

Original languageEnglish (US)
Article number47
JournalAntibodies
Volume8
Issue number3
DOIs
StatePublished - Sep 2019

Bibliographical note

Funding Information:
Funding: This work was supported in part by NCI P01 CA234228, administrative supplements to NIAID R37 AI064046 and P01GM091743, and by a grant from the University of Minnesota Academic Health Center. This research received assistance from the University of Minnesota’s Biorepository and Laboratory Services program, which is supported by the National Institutes of Health’s National Center for Advancing Translational Sciences, grant UL1TR002494. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health’s National Center for Advancing Translational Sciences. RSH is the Margaret Harvey Schering Land Grant Chair for Cancer Research and a Distinguished University McKnight Professor at the University of Minnesota, and an Investigator of the Howard Hughes Medical Institute.

Funding Information:
This work was supported in part by NCI P01 CA234228, administrative supplements to NIAID R37 AI064046 and P01GM091743, and by a grant from the University of Minnesota Academic Health Center. This research received assistance from the University of Minnesota?s Biorepository and Laboratory Services program, which is supported by the National Institutes of Health?s National Center for Advancing Translational Sciences, grant UL1TR002494. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health?s National Center for Advancing Translational Sciences. RSH is the Margaret Harvey Schering Land Grant Chair for Cancer Research and a Distinguished University McKnight Professor at the University of Minnesota, and an Investigator of the Howard Hughes Medical Institute.

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • APOBEC3B
  • Cancer biomarker
  • DNA cytosine deaminase
  • IHC assay
  • Rabbit monoclonal antibody

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