TY - JOUR
T1 - A rabbit model of pvr based on induction of endogenous cells and factors
AU - Frenzel, E. M.
AU - Walsh, A. W.
AU - Ncely, K. A.
AU - Cameron, J. D.
AU - Gregcrson, D. S.
PY - 1997/12/1
Y1 - 1997/12/1
N2 - Purpose. To design a new model of PVR in which the development of PVR was due to endogenous cells and factors. The proteolytic enzyme dispase was used to create conditions within the vitreous cavity that induced PVR. Dispase is commonly used for cell harvest and tissue dissociation, and is known to gently free cells from surrounding matrix. We examined whether an important aspect of the model was the formation of a retinal break, which would provide cells access to the vitreous cavity. Meflu-4ls. One eye of Dutch Belted rabbits was injected with dispase. Experimental conditions included; 1) subretmal injection of dispase, with or without a subsequent tear, 2) intravitreal injection of dispase, with or without endodiathermy holes, 3) a dose range of dispase of 0.003-1.0 units. Rabbits were examined and photographs were taken for up to 12 weeks post-op. Control rabbits received saline. Results. The injection of dispase into rabbit eyes led to PVR, whether dispase was injected subretinally or intravitreally. An increase in the severity of PVR was observed with increased doses of dispase. Evidence for PVR included visible preretinal membranes, distortion of myelin wings, vessel tortuosity, fixed retinal folds, and traction retinal detachment. Preretinal membranes were confirmed by histological examination. The induction of PVR by dispase occurred even in the absence of a retinal break. PVR did not develop in saline controls. Conclusions. PVR developed in rabbit eyes which received dispase, both with and without the formation of a retinal break. In the dispase model, PVR developed without the introduction of exogenous cells, such as dermal fibroblasts, but, rather, relied on the action of endogenous cells and factors. The dispase model of PVR provides an easy to perform procedure with little to no anterior inflammation, in which the lens remains clear and the pupil dilates well. As a result, excellent viewing and grading of the fundus is possible.
AB - Purpose. To design a new model of PVR in which the development of PVR was due to endogenous cells and factors. The proteolytic enzyme dispase was used to create conditions within the vitreous cavity that induced PVR. Dispase is commonly used for cell harvest and tissue dissociation, and is known to gently free cells from surrounding matrix. We examined whether an important aspect of the model was the formation of a retinal break, which would provide cells access to the vitreous cavity. Meflu-4ls. One eye of Dutch Belted rabbits was injected with dispase. Experimental conditions included; 1) subretmal injection of dispase, with or without a subsequent tear, 2) intravitreal injection of dispase, with or without endodiathermy holes, 3) a dose range of dispase of 0.003-1.0 units. Rabbits were examined and photographs were taken for up to 12 weeks post-op. Control rabbits received saline. Results. The injection of dispase into rabbit eyes led to PVR, whether dispase was injected subretinally or intravitreally. An increase in the severity of PVR was observed with increased doses of dispase. Evidence for PVR included visible preretinal membranes, distortion of myelin wings, vessel tortuosity, fixed retinal folds, and traction retinal detachment. Preretinal membranes were confirmed by histological examination. The induction of PVR by dispase occurred even in the absence of a retinal break. PVR did not develop in saline controls. Conclusions. PVR developed in rabbit eyes which received dispase, both with and without the formation of a retinal break. In the dispase model, PVR developed without the introduction of exogenous cells, such as dermal fibroblasts, but, rather, relied on the action of endogenous cells and factors. The dispase model of PVR provides an easy to perform procedure with little to no anterior inflammation, in which the lens remains clear and the pupil dilates well. As a result, excellent viewing and grading of the fundus is possible.
UR - http://www.scopus.com/inward/record.url?scp=33749216709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749216709&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33749216709
SN - 0146-0404
VL - 38
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 4
ER -