A QIL1 variant associated with ventricular arrhythmias and sudden cardiac death in the juvenile rhodesian ridgeback dog

Kathryn M. Meurs, Steven G Friedenberg, Natasha J. Olby, Julia Condit, Jess Weidman, Steve Rosenthal, G. Diane Shelton

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The QIl1 gene produces a component of the Mitochondrial Contact Site and Cristae Organizing System that forms and stabilizes mitochondrial cristae junctions and is important in cellular energy production. We previously reported a family of Rhodesian Ridgebacks with cardiac arrhythmias and sudden cardiac death. Here, we performed whole genome sequencing on a trio from the family. Variant calling was performed using a standardized bioinformatics approach. Variants were filtered against variants from 247 dogs of 43 different breeds. High impact variants were validated against additional affected and unaffected dogs. A single missense G/A variant in the QIL1 gene was associated with the cardiac arrhythmia (p < 0.0001). The variant was predicted to change the amino acid from conserved Glycine to Serine and to be deleterious. Ultrastructural analysis of the biceps femoris muscle from an affected dog revealed hyperplastic mitochondria, cristae rearrangement, electron dense inclusions and lipid bodies. We identified a variant in the Q1l1 gene resulting in a mitochondrial cardiomyopathy characterized by cristae abnormalities and cardiac arrhythmias in a canine model. This natural animal model of mitochondrial cardiomyopathy provides a large animal model with which to study the development and progression of disease as well as genotypic phenotypic relationships.

Original languageEnglish (US)
Article number168
JournalGenes
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Sudden Cardiac Death
Cardiac Arrhythmias
Dogs
Cardiomyopathies
Animal Models
Genes
Inclusion Bodies
Computational Biology
Glycine
Serine
Disease Progression
Canidae
Mitochondria
Genome
Electrons
Amino Acids
Muscles

Keywords

  • Arrhythmia
  • Mitochondrial cristae
  • QIL1
  • Rhodesian ridgeback

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

A QIL1 variant associated with ventricular arrhythmias and sudden cardiac death in the juvenile rhodesian ridgeback dog. / Meurs, Kathryn M.; Friedenberg, Steven G; Olby, Natasha J.; Condit, Julia; Weidman, Jess; Rosenthal, Steve; Shelton, G. Diane.

In: Genes, Vol. 10, No. 2, 168, 01.02.2019.

Research output: Contribution to journalArticle

Meurs, Kathryn M. ; Friedenberg, Steven G ; Olby, Natasha J. ; Condit, Julia ; Weidman, Jess ; Rosenthal, Steve ; Shelton, G. Diane. / A QIL1 variant associated with ventricular arrhythmias and sudden cardiac death in the juvenile rhodesian ridgeback dog. In: Genes. 2019 ; Vol. 10, No. 2.
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