A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses

Touraj Aligholipour Farzani, Angela Chov, Alon Herschhorn

Research output: Contribution to journalArticlepeer-review

Abstract

We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the vsv-g gene. To prepare rcVSVs, we replace the vsv-g with a specific env-encoding gene, transfect cells with multiple plasmids for production of the genomic RNA and viral proteins, and rescue replication-competent viruses.

Original languageEnglish (US)
Pages (from-to)100209
JournalSTAR protocols
Volume1
Issue number3
DOIs
StatePublished - Dec 18 2020

Bibliographical note

© 2020 The Author(s).

PubMed: MeSH publication types

  • Journal Article

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