A proteomic analysis of differentiating dopamine neurons derived from human embryonic stem cells

Joohyun Ryu, Byoung Chul Park, Do Hee Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Human embryonic stem cells (hESC) are being exploited for potential use in cell transplantation due to their capacity for self-renewal and pluripotency. Dopamine (DA) neurons derived from hESC represent a promising source of cell replacement therapy for Parkinson’s disease (PD). While gene expression on the transcriptome level has been extensively studied, limited information is available for the proteome-level changes associated with DA neuron differentiation. Here we analyzed the proteome of differentiating DA neurons to search for the potential biomarkers to assess the efficiency of differentiation. Although the proteome profile of DA neurons did not exhibit significant changes, a number of cytoskeletal proteins including nuclear lamin, tropomyosin 1, and myosin light chain 1 were specifically up-regulated during differentiation. Expression analysis of the respective genes was also consistent with the proteome results. In addition, these differentially expressed proteins form protein interaction network with several PD-related proteins suggesting that they may play roles in PD pathogenesis as well as the maturation of DA neurons.

Original languageEnglish (US)
Pages (from-to)219-227
Number of pages9
JournalAnimal Cells and Systems
Volume23
Issue number3
DOIs
StatePublished - Jun 2019

Bibliographical note

Funding Information:
This work was supported by a research grant from the Seoul Women?s University (2019). We are grateful to Prof. Yong-Sung Lee (Hanyang University) for generous providing of the dopaminergic neurons derived from H9 cells and the results of microarray experiments.

Funding Information:
This work was supported by a research grant from the Seoul Women’s University (2019).

Publisher Copyright:
© 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • cytoskeletal proteins
  • dopamine neuron
  • Human embryonic stem cells
  • proteome

PubMed: MeSH publication types

  • Journal Article

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