A primer for morpholino use in zebrafish

Brent R. Bill, Andrew M. Petzold, Karl J. Clark, Lisa A Schimmenti, Stephen C. Ekker

Research output: Contribution to journalReview articlepeer-review

287 Scopus citations

Abstract

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalZebrafish
Volume6
Issue number1
DOIs
StatePublished - Mar 1 2009

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