Abstract
Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-77 |
| Number of pages | 9 |
| Journal | Zebrafish |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2009 |