A preliminary survey of lichen associated eukaryotes using pyrosequencing

Scott T Bates, Donna Berg-Lyons, Christian L. Lauber, William A. Walters, Rob Knight, Noah Fierer

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Although various eukaryotic organisms, such as arthropods, endolichenic/lichenicolous fungi, and nematodes, have been isolated from lichens, the diversity and structure of eukaryotic communities associated with lichen thalli has not been well studied. In addressing this knowledge gap, we used bar-coded pyrosequencing of 18S rRNA genes to survey eukaryotes associated with thalli of three different lichen species. In addition to revealing an expected high abundance of lichen biont-related 18S genes, sequences recovered in our survey showed non-biont fungi from the Ascomycota also have a substantial presence in these thalli. Our samples additionally harboured fungi representing phyla (Blastocladiomycota, Chytridiomycota) that have not been isolated previously from lichens; however, their very low abundance indicates an incidental presence. The recovery of Alveolata, Metazoa, and Rhizaria sequences, along with recent work revealing the considerable bacterial diversity in these same samples, suggests lichens function as minute ecosystems in addition to being symbiotic organisms.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalLichenologist
Volume44
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

eukaryote
lichen
lichens
eukaryotic cells
Chytridiomycota
thallus
fungus
fungi
Animalia
gene
organisms
Ascomycota
arthropod
nematode
arthropods
Nematoda
ribosomal RNA
nucleotide sequences
sampling
ecosystems

Keywords

  • Ascomycota
  • bacteria
  • endolichenic/lichenicolous fungi
  • invertebrates

Cite this

Bates, S. T., Berg-Lyons, D., Lauber, C. L., Walters, W. A., Knight, R., & Fierer, N. (2012). A preliminary survey of lichen associated eukaryotes using pyrosequencing. Lichenologist, 44(1), 137-146. https://doi.org/10.1017/S0024282911000648

A preliminary survey of lichen associated eukaryotes using pyrosequencing. / Bates, Scott T; Berg-Lyons, Donna; Lauber, Christian L.; Walters, William A.; Knight, Rob; Fierer, Noah.

In: Lichenologist, Vol. 44, No. 1, 01.01.2012, p. 137-146.

Research output: Contribution to journalArticle

Bates, ST, Berg-Lyons, D, Lauber, CL, Walters, WA, Knight, R & Fierer, N 2012, 'A preliminary survey of lichen associated eukaryotes using pyrosequencing', Lichenologist, vol. 44, no. 1, pp. 137-146. https://doi.org/10.1017/S0024282911000648
Bates ST, Berg-Lyons D, Lauber CL, Walters WA, Knight R, Fierer N. A preliminary survey of lichen associated eukaryotes using pyrosequencing. Lichenologist. 2012 Jan 1;44(1):137-146. https://doi.org/10.1017/S0024282911000648
Bates, Scott T ; Berg-Lyons, Donna ; Lauber, Christian L. ; Walters, William A. ; Knight, Rob ; Fierer, Noah. / A preliminary survey of lichen associated eukaryotes using pyrosequencing. In: Lichenologist. 2012 ; Vol. 44, No. 1. pp. 137-146.
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