A precerebellin-like protein is part of the acute phase response in rainbow trout, Oncorhynchus mykiss

Lena Gerwick, Wendy S. Reynolds, Christopher J. Bayne

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The acute phase response (APR) has a long evolutionary history, but it remains to be characterized fully in lower vertebrates. To study the acute phase proteins of a teleost, rainbow trout (Oncorhynchus mykiss), we induced an APR by injecting Vibrio bacterin emulsified in FIA. In samples taken over the next 3 weeks, the total plasma protein profile changed consistently as seen in one and two-dimensional SDS PAGE. One 18.1 kD upregulated protein was isolated from 2D gels and an N-terminal sequence obtained. Using reverse transcriptase-PCR, a 700 bp cDNA sequence was amplified. The sequence is 53% similar at the amino acid level with rat precerebellin (regions aa 42-184 from trout and aa 89-224 precerebellin), and 46% similar with the globular portion of the human B chain of the first complement component C1q. However, it lacks the collagen portion of C1q with its characteristic Gly-X-Y repeats. The isolated protein seems to be involved in the inflammatory response but its physiological function is unknown. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)597-607
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume24
Issue number6-7
DOIs
StatePublished - Sep 1 2000
Externally publishedYes

Keywords

  • 2D gel
  • Acute phase protein
  • C1q B chain
  • Cerebellin
  • Full length cDNA sequence
  • Oncorhynchus mykiss
  • Plasma
  • Precerebellin

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