Abstract
Pheromonally-mediated trapping is currently being developed for use in sea lamprey control in the Laurentian Great Lakes. To identify and test lamprey pheromones a practical procedure was needed to isolate relatively large quantities of pheromone from lamprey holding water. The present study developed such a technique. It employs Amberlite XAD7HP, an adsorbent resin which we found can extract over 80% of the sea lamprey migratory pheromone from larval holding water at low cost and with relative ease. This technique allowed its to collect tens of milligrams of all three components of the sea lamprey migratory pheromone, eventually permitting both identification and successful field testing. This technique might also be used to collect pheromones released by other species of fish.
Original language | English (US) |
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Pages (from-to) | 832-838 |
Number of pages | 7 |
Journal | Journal of Great Lakes Research |
Volume | 32 |
Issue number | 4 |
DOIs | |
State | Published - 2006 |
Bibliographical note
Funding Information:We thank the staff of the Hammond Bay Biological Station (U.S. Geological Survey) for assistance, access to their facilities, and larval lampreys. Thanks also to Tom Krick and Leeann Higgins of the Mass Spectrometry Consortium for the Life Sciences at the University of Minnesota. George Spangler, LeeAnn Higgins, and Matt Wisnioski kindly read early versions of this manuscript. Funding was provided by both the Great Lakes Fishery Commission and the Minnesota Agricultural Experiment Station.
Keywords
- Invasive fishes
- Petromyzonamine disulfate
- Petromyzonol sulfate
- Petromyzosterol disulfate
- Pheromone
- Sea lamprey