A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

Christopher J. Stenland; Lev G. Lis; Frederick J. Schendel; Nicholas J. Hahn; Mary A. Smart; Amy L. Miller; Marc G. Von Keitz; Vadim J. Gurvich

doi:10.1021/op3003294

A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

Christopher J. Stenland
, Lev G. Lis
, Frederick J. Schendel
, Nicholas J. Hahn
, Mary A. Smart
, Amy L. Miller
, Marc G. Von Keitz
, Vadim J. Gurvich

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.

Original language	English (US)
Pages (from-to)	265-272
Number of pages	8
Journal	Organic Process Research and Development
Volume	17
Issue number	2
DOIs	https://doi.org/10.1021/op3003294
State	Published - Feb 15 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Publisher link

10.1021/op3003294

Find It

Find It at U of M Libraries

Cite this

@article{4ef92d3cc653480bab322fbc462f294f,

title = "A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z",

abstract = "A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79\% purification yield, and >98\% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.",

author = "Stenland, \{Christopher J.\} and Lis, \{Lev G.\} and Schendel, \{Frederick J.\} and Hahn, \{Nicholas J.\} and Smart, \{Mary A.\} and Miller, \{Amy L.\} and \{Von Keitz\}, \{Marc G.\} and Gurvich, \{Vadim J.\}",

year = "2013",

month = feb,

day = "15",

doi = "10.1021/op3003294",

language = "English (US)",

volume = "17",

pages = "265--272",

journal = "Organic Process Research and Development",

issn = "1083-6160",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

AU - Stenland, Christopher J.

AU - Lis, Lev G.

AU - Schendel, Frederick J.

AU - Hahn, Nicholas J.

AU - Smart, Mary A.

AU - Miller, Amy L.

AU - Von Keitz, Marc G.

AU - Gurvich, Vadim J.

PY - 2013/2/15

Y1 - 2013/2/15

N2 - A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.

AB - A scalable and reliable manufacturing process for nikkomycin Z HCl on a scale of 170 g has been developed and optimized. The process is characterized by a 2.3 g/L fermentation yield, 79% purification yield, and >98% relative purity of the final product. This method is suitable for further scale-up and cGMP production. The Streptomyces tendae ΔNikQ strain developed during the course of this study is superior to any previously reported strain in terms of higher yield and purity of nikkomycin Z.

UR - https://www.scopus.com/pages/publications/84874084416

UR - https://www.scopus.com/pages/publications/84874084416#tab=citedBy

U2 - 10.1021/op3003294

DO - 10.1021/op3003294

M3 - Article

AN - SCOPUS:84874084416

SN - 1083-6160

VL - 17

SP - 265

EP - 272

JO - Organic Process Research and Development

JF - Organic Process Research and Development

IS - 2

ER -

A practical and scalable manufacturing process for an antifungal agent, nikkomycin Z

Abstract

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this