A porcine enterovirus G associated with enteric disease contains a novel papain-like cysteine protease

Todd P. Knutson, Binu T. Velayudhan, Douglas G. Marthaler

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Identification of unknown pathogens in pigs displaying enteric illness is difficult due to the large diversity of bacterial and viral species found within faecal samples. Current methods often require bacterial or viral isolation, or testing only a limited number of known species using quantitative PCR analysis. Herein, faeces from two 25-day-old piglets with diarrhoea from Texas, USA, were analysed by metagenomic next-generation sequencing to rapidly identify possible pathogens. Our analysis included a bioinformatics pipeline of rapid short-read classification and de novo genome assembly which resulted in the identification of a porcine enterovirus G (EV-G), a complete genome with substantial nucleotide differences (>30 %) among current sequences, and a novel non-structural protein similar in sequence to the Torovirus papain-like cysteine protease (PLpro). This discovery led to the identification and circulation of an EV-G with a novel PLpro in the USA that has not been previously reported.

Original languageEnglish (US)
Article number000799
Pages (from-to)1305-1310
Number of pages6
JournalJournal of General Virology
Issue number6
StatePublished - Jun 2017

Bibliographical note

Funding Information:
This work was supported by Marthaler Start-up fund and the University of Minnesota Veterinary Diagnostic Laboratory.

Publisher Copyright:
© 2017, Microbiology Society. All rights reserved.


  • Enterovirus G
  • Metagenomics
  • Papain-like cysteine protease
  • Picornavirus
  • Porcine
  • Torovirus


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