A plasmid model to study genetic recombination in yeast

Janet E. Embretson, Dennis M Livingston

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Chimeric plasmids have been constructed containing two heteroallelic mutant copies of the yeast HIS3 gene as an inverted repetition. Intramolecular exchange events between these two allelic mutant copies are capable of generating a wild-type allele. Plasmids containing two mutant heteroalleles have been transformed into appropriate his3- yeast strains, and the frequency of exchange events generating His+ prototrophs has been measured during mitotic division. After 20 generations of growth under nonselective conditions, between 0.1 and 1 % of the transformed yeast cells become His+ prototrophs. This percentage decreases at least ten-fold in a strain with a rad52 mutation. Plasmid molecules having undergone exchange events have been isolated from yeast cells and have been examined after transfer to Escherichia coli. Physical examination shows that less than 10 % of the plasmids having undergone genetic exchange have also undergone an internal reciprocal recombination event as evidenced by reorientation of linked restriction sites. The remainder of the plasmids having undergone genetic exchange do not exhibit reciprocal recombination. Characterization of the individual allelic copies within a plasmid having undergone exchange reveals that in 24 of 25 examples only one of the two HIS3 copies has become wild type, and that either copy is equally likely to become wild type. We conclude that the model plasmid we have constructed undergoes intramolecular genetic exchange events and will be useful for studying genetic recombination.

Original languageEnglish (US)
Pages (from-to)293-302
Number of pages10
JournalGene
Volume29
Issue number3
DOIs
StatePublished - Sep 1984

Keywords

  • HIS3 heteroalleles
  • Saccharomyces cerevisiae
  • gene conversion
  • mitotic recombination
  • reciprocal recombination

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