To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.
Bibliographical noteFunding Information:
We thank Jack R. Staunton and Denis Wirtz for taking leadership in preparing this manuscript, and we thank Thea D. Tlsty and Barbara L. Hempstead for their input on the choice of cell lines for this project. This work was supported by the following grants from the United States National Cancer Institute: U54CA143862 to P.C.W.D., U54CA143876 to M.L.S., U54CA143798 to F.M., U54CA143970 to R.A.G., U54CA143868 to D.W., U54CA143874 to A.V.O., U54CA143837 to M.F., U54CA143869 to T.V.O., U54CA143803 to R.H.A., U54CA143906 to P.K., U54CA143836 to J.T.L., and U54CA143907 to W.D.H. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health.