A physical map of the 1-gigabase bread wheat chromosome 3B

Etienne Paux, Pierre Sourdille, Jérôme Salse, Cyrille Saintenac, Frédéric Choulet, Philippe Leroy, Abraham Korol, Monika Michalak, Shahryar Kianian, Wolfgang Spielmeyer, Evans Lagudah, Daryl Somers, Andrzej Kilian, Michael Alaux, Sonia Vautrin, Hélène Bergès, Kellye Eversole, Rudi Appels, Jan Safar, Hana SimkovaJaroslav Dolezel, Michel Bernard, Catherine Feuillet

Research output: Contribution to journalArticlepeer-review

301 Scopus citations

Abstract

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalScience
Volume322
Issue number5898
DOIs
StatePublished - Oct 3 2008

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